Invitrogen™ CD138 (Syndecan-1) Monoclonal Antibody (MI15), PE-eFluor™ 610, eBioscience™, Invitrogen™

Catalog No.	Quantity
61-138-842	100 Tests

Catalog No. 61-138-842 Supplier Invitrogen™ Supplier No. 61138842

Description

Mouse Monoclonal Antibody

Description: This MI15 monoclonal antibody reacts with human CD138, also known as syndecan-1. CD138 is a transmembrane protein containing chondroitin sulfate and heparin sulfate moieties responsible for binding to the extracellular matrix components. CD138 is not expressed by mature B cells but is present on pre-B cells and the finally differentiated plasma cells. Applications Reported: This MI15 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MI15 antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This may be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red). Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No. 00-8222-49) (100 μL of cell sample + 100 μL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product No.

CD138 (syndecan 1) is a transmembrane proteoglycan that can bind a variety of cytokines and modulate their activity, as well as the activity of extracellular matrix components and influence many developmental processes. CD138 is expressed mainly in differentiating keratinocytes and is transiently upregulated in all layers of the epidermis upon tissue injury. It is also highly expressed on plasma cells and can be detected even on fibroblasts, vascular smooth muscle cells and endothelial cells. Up-regulation and down-regulation of CD138 on the cell surface often correlates with the gain of cancerous characteristics. Serum levels of the shedded soluble sCD138 are used as a prognostic factor of cancerogenesis.

Specifications

• Antigen: CD138 (Syndecan-1)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: MI15
• Concentration: 5 μL/Test
• Conjugate: PE-eFluor 610
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: Sdc1
• Gene Accession No.: P18827
• Gene Alias: AA408134; AA409076; CD antigen 138; CD138; CD138 antigen; heparan sulfate proteoglycan fibroblast growth factor receptor; HSPG; sCD138; SDC; Sdc1; soluble CD138; Sstn; syn-1; Synd; SYND1; Synd-1; SYNDECA; syndecan; syndecan 1; syndecan proteoglycan 1; Syndecan1; syndecan-1; synstatin
• Gene Symbols: Sdc1
• Host Species: Mouse
• Immunogen: U-266 cell line.
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 6382
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ