Invitrogen™ CD14 Monoclonal Antibody (MEM-15), NovaFluor™ Blue 530, eBioscience™, Invitrogen™

Catalog No.	Quantity
H007T03B02A	100 Tests
H007T02B02A	25 Tests

Catalog No. H007T03B02A Supplier Invitrogen™ Supplier No. H007T03B02A

Description

Mouse Monoclonal Antibody

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Specifications

• Antigen: CD14
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: MEM-15
• Concentration: 1.0 μg/Test
• Conjugate: NovaFluor Blue 530
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: Cd14
• Gene Accession No.: P08571
• Gene Alias: CD 14; CD14; CD14 antigen; CD14 molecule; cd14 monocyte; lipopolysaccharide receptor; lipoprotein receptor; LPSR antibody; monocyte differentiation antigen CD14; Monocyte differentiation antigen CD14, membrane-bound form; Monocyte differentiation antigen CD14, urinary form; monocyte differentiation antigen CD14; LOW QUALITY PROTEIN: monocyte differentiation antigen CD14; myeloid cell-specific leucine-rich glycoprotein; myeloid membrane glycoprotein precursor; sCD14; soluble CD14
• Gene Symbols: Cd14
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 929
• Target Species: Human, Monkey
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Format: Liquid
• Isotype: IgG1

Frequently Asked Questions (FAQs)

What fixation methods are compatible with NovaFluor dyes?

NovaFluor conjugates have been tested to be stable for up to 14 days on cells fixed with 1% formalin, 2% paraformaldehyde, eBioscience IC Fixation Buffer (Cat. No. 00-8222-49), or eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00) when stored at 2-8 degrees C in the dark.

NovaFluor dyes can also be used as a surface stain prior to methanol fixation.