CD14 Mouse anti-Baboon, Cynomolgus, Human, Rhesus, Brilliant Ultraviolet 737, Clone: M5E2, BD Horizon™

Mouse Monoclonal Antibody

Supplier:  BD Biosciences 612763

Catalog No. BDB612763

Description

The M5E2 monoclonal antibody specifically binds to CD14, a 53–55 kDa glycosylphosphatidylinositol (GPI)-anchored single chain glycoprotein expressed at high levels on monocytes. Additionally, the anti-CD14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some Langerhans cells. CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB.

The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).

Specifications

• Antigen: CD14
• Classification: Monoclonal
• Conjugate: Brilliant Ultraviolet 737
• Host Species: Mouse
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Target Species: Baboon, Cynomolgus, Human, Rhesus Monkey
• Isotype: IgG2a κ

• Applications: Flow Cytometry
• Clone: M5E2
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Immunogen: Human CD14 Protein
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.