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CD14 Mouse anti-Human, Clone: 61D3, eBioscience™ Non-distribution product as customer accommodation.

Mouse Monoclonal Antibody

$49.98 - $101.00

Specifications

Antigen CD14
Clone 61D3
Target Species Human
Conjugate Unconjugated
Isotype IgG1, kappa
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-124-34 Non-distribution product as customer accommodation.
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Invitrogen
14014980
25 μg
Each for $49.98
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50-124-35 Non-distribution product as customer accommodation.
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Invitrogen
14014982
100 μg
Each for $101.00
Add to cart
 
Description

Description

Description: The 61D3 monoclonal antibody reacts with human CD14, a 53-55 kDa GPI-linked glycoprotein. CD14 is expressed on monocytes, interfollicular macrophages and some dendritic cells. Complexes of LPS and LBP (LPS-Binding Protein) bind with high affinity to monocytes through the surface CD14. Applications Reported: The 61D3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of formalin-fixed paraffin embedded human tissue. 61D3 has also been reported for in vitro functional studies. (Please use Functional Grade Purified 61D3, cat. 16-0149, in functional assays, and fluorochrome-conjugated 61D3 is recommended for use in flow cytometry.). Applications Tested: The 61D3 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The 61D3 antibody has also been tested by immunohistochemistry of formalin-fixed paraffin embedded human tissue using low pH antigen retreival and can be used at less than or equal to 20 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.
Specifications

Specifications

CD14
Human
IgG1, kappa
Monoclonal
Liquid
Antibody
4° C
61D3
Unconjugated
Flow Cytometry, Functional Assay, Immunohistochemistry (Paraffin)
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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