Invitrogen™ CD141 Monoclonal Antibody (JAA17), Brilliant Violet™ 480, eBioscience™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
41-414-1941	Invitrogen™ 414141941	25 Tests
41-414-1942	Invitrogen™ 414141942	100 Tests

Description

Description This JAA17 monoclonal antibody recognizes human CD141 also known as thrombomodulin, fetomodulin, or BDCA-3. In humans, CD141 is commonly used as a marker of cross-presenting dendritic cells in both lymphoid and non-lymphoid tissues. Retinoic acid has been shown to upregulate CD141 expression on human monocyte-derived dendritic cells and primary human dendritic cells. The JAA17 antibody will recognize CD141 on cells that have been formaldehyde-fixed and permeabilized. Applications Tested This JAA17 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Blocking Buffers When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401</a>) or Brilliant Stain Buffer (Product # 00-4409-75</a>) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer or Brilliant Stain Buffer for more information. Fixation Samples can be stored i...

Thrombomodulin (TM) is also known as Fetomodulin (FM), endothelial anticoagulant protein, or glycoprotein P112. Thrombomodulin contains six repeated domains homologous with epidermal growth factor (EGF) and an amino terminal domain homologous to lectin-like protein. Through its accelerated activation of protein C, synthesis of TM is one of several mechanisms important in maintaining thrombo-resistance and thus reducing clot formation on the surface of the endothelial cells. Immunohistochemical staining of formalin-fixed tissue for TM has been used for the study of a variety of vascular tumors, meningiomas and choriocarcinomas.

Specifications

• Antigen: CD141
• Concentration: 5 μL/Test
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 480
• Host Species: Mouse
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P07204
• Gene ID (Entrez): 7056
• Primary or Secondary: Primary
• Purification Method: Affinity chromatography

• Clone: JAA17
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Classification: Monoclonal
• Form: Liquid
• Isotype: IgG2a κ
• Target Species: Human
• Gene: THBD
• Gene Alias: AHUS6; AI385582; BDCA3; BDCA-3; CD141; CD141 antigen; DC141; fetomodulin; sCD141; snoRNA MBII-339; soluble CD141; THBD; THPH12; THRM; thrombomdulin; thrombomodulin; thrombomodulin precursor; TM
• Gene Symbols: THBD
• Product Type: Antibody