Invitrogen™ CD142 Monoclonal Antibody (HTF-1), PE, eBioscience™

Catalog No.	Quantity
50-112-2272	100 Tests

Catalog No. 50-112-2272 Supplier Invitrogen™ Supplier No. 12142942

Description

Mouse Monoclonal Antibody

Description: This HTF-1 monoclonal antibody reacts with human CD142, which is also known as Tissue Factor. Expression of this type I transmembrane glycoprotein on endothelial cells, monocytes, macrophages, and platelets can be induced by inflammatory mediators (e.g., LPS, IL-1b, TNFa, PMA, or endotoxin). On the other hand, CD142 is expressed constitutively by some tumor cells (e.g., lung, pancreatic, breast, and colon) and non-immune tissues such as the vasculature, central nervous system, kidney, epithelia, and placenta. Studies have also suggested that CD142 exists as a soluble form that circulates in blood. CD142 initiates blood coagulation by associating with and activating the circulating factors VII and VIIa. The HTF-1 antibody has been reported to exhibit blocking activity. Applications Reported: This HTF-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HTF-1 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described.

Specifications

• Antigen: CD142
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: HTF-1
• Concentration: 5 μL/Test
• Conjugate: PE
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: F3
• Gene Accession No.: P13726
• Gene Alias: AA409063; CD142; Cf3; Cf-3; Coagulation factor III; coagulation factor III (thromboplastin, tissue factor); coagulation factor III, tissue factor; F3; TF; TFA; Thromboplastin; Tissue factor
• Gene Symbols: F3
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2152
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ