Invitrogen™ CD144 (VE-cadherin) Monoclonal Antibody (eBioBV13 (BV13)), Functional Grade, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-144-67	500 μg

Catalog No. 50-144-67 Supplier Invitrogen™ Supplier No. 16144185

Description

Rat Monoclonal Antibody

Description: The BV13 monoclonal antibody reacts with mouse VE-Cadherin (CD144). VE-Cadherin is a 120 kDa member of the type II Cadherin family, characterized by the presence of 5 extracellular cadherin domains (ECD), and anchored to the actin cytoskeleton through their cytoplasmic tail. VE-Cadherin mediates homophilic adhesion between neighbouring endothelial cells and is localized within specialized structures at cell-cell contacts, called adherens junctions. VE-Cadherin is expressed constitutively throughout the entire vasculature, and is required for numerous endothelial cell functions including migration, survival, contact-dependent growth inhibition and endothelial cell assembly into tubular structures. Furthermore, it is thought that VE-Cadherin+CD45- cells from the yolk sac or aorta-gonad-mesonephros (AGM) have the potential to give rise to hematopoietic cells. Applications Reported: This eBioBV13 (BV13) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, immunoblotting (WB), and immunohistology staining of frozen tissue sections. Intravenous injection of BV13 has been shown to induce a concentration- and time-dependent increase in vascular permeability in the heart and lungs. Applications Tested: This eBioBV13 (BV13) antibody has been tested by flow cytometric analysis of bEnd.3 cells. This can be used at less than or equal to 1 μg per test.

VE-cadherin is a member of the cadherin superfamily that is located in a six-cadherin cluster in a region on the long arm of chromosome 16 and is involved in loss of heterozygosity events in breast and prostate cancer. VE-cadherin protein is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, VE-cadherin may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. An alternative splice variant has been described but the full length sequence of VE-cadherin has not been determined.

Specifications

• Antigen: CD144 (VE-cadherin)
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Immunoprecipitation, Western Blot
• Classification: Monoclonal
• Clone: eBioBV13 (BV13)
• Concentration: 1 mg/mL
• Conjugate: Functional Grade
• Formulation: PBS with no preservative; pH 7.2
• Gene: CDH5
• Gene Accession No.: P55284
• Gene Alias: 7B4; 7B4 antigen; 7B4/cadherin-5; AA408225; cadherin 5; cadherin 5, type 2 (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular endothelium); cadherin 5, type 2, VE-cadherin (vascular epithelium); cadherin-5; cadherin-5; cd144 antigen; VE cadherin; Cd144; cd144 antigen; CDH5; cell-cell adhesion protein; endothelial-specific cadherin; vascular endothelial cadherin; vascular endothelial cadherin precursor; Vec; VEcad; VE-Cad; VE-cadherin; VECD
• Gene Symbols: CDH5
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 500 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 12562
• Target Species: Mouse
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

For Research Use Only.