Invitrogen™ CD146 Recombinant Rabbit Monoclonal Antibody (044), PE

Catalog No.	Quantity
PIMA546637	100 Tests

Catalog No. PIMA546637 Supplier Invitrogen™ Supplier No. MA546637

Description

Rabbit Recombinant Monoclonal Antibody

Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD146 (Met1-Gly559) and conjugated with PE under optimum conditions, the unreacted PE was removed. Storage: This antibody can be stored at 2C-8C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD146, also known as MCAM (Melanoma Cell Adhesion Molecule), is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is heavily glycosylated, with more than 50% of its mass derived from carbohydrates. CD146 is primarily expressed on endothelial cells at cell-cell junctions, playing a crucial role in cell adhesion and the cohesion of the endothelial monolayer in vascular tissue. In addition to endothelial cells, CD146 is expressed on bone marrow fibroblasts, mesenchymal stromal cells, endometrial stromal cells, and some tumors, particularly melanoma. It has also been observed on a subset of circulating T cells and activated lymphocytes, but not on circulating endothelial cells, as indicated by the absence of other endothelial markers such as CD31 and CD51/61. CD146 functions as a calcium-independent cell adhesion molecule and may also act as a signal transduction molecule. It is involved in the recruitment of Fyn kinase and the subsequent tyrosine phosphorylation of intracellular proteins, which are important for actin cytoskeleton assembly. This signaling role includes triggering tyrosine phosphorylation of FYN and PTK2 and causing a transient increase in intracellular calcium concentration. In the context of cancer, CD146 expression may facilitate the interaction of melanoma cells with the vascular system, enhancing hematogenous tumor spread. Additionally, CD146 could serve as an adhesion molecule active in neural crest cells during embryonic development, highlighting its diverse roles in both normal physiological processes and disease states.

Specifications

• Antigen: CD146
• Applications: Flow Cytometry, Immunocytochemistry
• Classification: Recombinant Monoclonal
• Clone: 044
• Concentration: 100 μg/mL
• Conjugate: PE
• Formulation: PBS with 0.5% BSA and 0.1% sodium azide
• Gene: MCAM
• Gene Accession No.: P43121
• Gene Alias: 1-gicerin; AV025631; CD146; CD149; Cell surface glycoprotein MUC18; cell surface glycoprotein P1H12; Gicerin; HEMCAM; l-gicerin; l-gicerin precursor; l-gicerin protein; LOW QUALITY PROTEIN: cell surface glycoprotein MUC18; MCAM; melanoma adhesion molecule; melanoma cell adhesion molecule; Melanoma-associated antigen A32; melanoma-associated antigen MUC18; Muc18; mucin-18; s-endo; S-endo 1 endothelial-associated antigen; s-gicerin; s-gicerin precursor
• Gene Symbols: MCAM
• Host Species: Rabbit
• Immunogen: Recombinant protein ecoding Human CD146 (Met1-Gly559).
• Purification Method: Protein A
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 4162
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov