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Invitrogen™ CD15 Monoclonal Antibody (MMA), Alexa Fluor™ Plus 594

Catalog No. 755015894
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75-501-5894 500 μL
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Catalog No. 75-501-5894 Supplier Invitrogen™ Supplier No. 755015894
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Description

Mouse Monoclonal Antibody

Description: The MMA monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative. Applications Reported: This MMA antibody has been reported for use in flow cytometric analysis and immunohistochemical staining of paraffin-embedded tissue. Applications Tested: This MMA antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tissue with high pH antigen retrieval at 1 μg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

CD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin's disease.
TRUSTED_SUSTAINABILITY

Specifications

Antigen CD15
Applications Immunohistochemistry (Paraffin), Multiplex Immunohistochemistry
Classification Monoclonal
Clone MMA
Concentration 0.2 mg/mL
Conjugate Alexa Fluor Plus 594
Formulation PBS with 0.5% BSA, 10% proprietary stabilizer and 0.05% sodium azide; pH 7.2
Gene FUT4
Gene Accession No. P22083
Gene Alias FUTIV; Lewis X; SSEA-1
Gene Symbols FUT4
Host Species Mouse
Purification Method Affinity chromatography
Quantity 500 μL
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 2526
Target Species Human
Content And Storage 4°C, store in dark
Product Type Antibody
Form Liquid
Isotype IgM
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