Invitrogen™ CD154 (CD40 Ligand) (soluble) Polyclonal Antibody, PeproTech®, Invitrogen™

Catalog No.	Quantity
500P142G50	50 μg
500P142G100	100 μg
500P142G1MG	1 mg

Catalog No. 500P142G50 Supplier Invitrogen™ Supplier No. 500P142G50UG

Description

Goat Polyclonal Antibody

AA Sequence of recombinant protein: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL. Preparation: Produced from sera of goats immunized with highly pure Recombinant Human sCD40 Ligand. Anti-Human sCD40 Ligand-specific antibody was purified by affinity chromatography employing an immobilized Human sCD40 Ligand matrix. Sandwich ELISA: To detect Human sCD40 Ligand by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human sCD40 Ligand (500-P142GBt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human sCD40 Ligand. Western Blot: To detect Human sCD40 Ligand by Western Blot analysis this antibody can be used at a concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human sCD40 Ligand is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

CD40 Ligand (CD40-L), or CD154, is a membrane glycoprotein and differentiation antigen expressed on the surface of T cells. The CD40 Ligand stimulates B cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40 Ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL-2.

Specifications

• Antigen: CD154 (CD40 Ligand) (soluble)
• Applications: ELISA, Western Blot
• Classification: Polyclonal
• Concentration: 0.1-1.0 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with no preservative
• Gene: CD40LG
• Gene Accession No.: P29965
• Gene Alias: CD154; CD40 antigen ligand; CD40 ligand; CD40 ligand CD154; CD40 ligand, membrane form; CD40 ligand, soluble form; CD40L; CD40-L; Cd40lg; gp39; hCD40L; H-CD-40-L; HIGM1; IGM; IMD3; Ly62; Ly-62; M-CD-40-L; RP23-153G22.3; T-B cell-activating molecule; T-BAM; T-cell antigen Gp39; TNF-related activation protein; TNFSF5; TRAP; tumor necrosis factor (ligand) superfamily member 5; tumor necrosis factor (ligand) superfamily, member 5; tumor necrosis factor ligand superfamily member 5
• Gene Symbols: CD40LG
• Host Species: Goat
• Immunogen: E.coli-derived Recombinant Human sCD40 Ligand.
• Purification Method: Antigen affinity chromatography
• Quantity: 50 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 959
• Target Species: Human
• Content And Storage: -20°C
• Product Type: Antibody
• Form: Lyophilized