Invitrogen™ CD16/CD32 Monoclonal Antibody (FRC-4G8)

Catalog No.	Quantity
50-113-8121	2 mL

Catalog No. 50-113-8121 Supplier Invitrogen™ Supplier No. MFCR004

Description

Rat Monoclonal Antibody

CD16/CD32 Monoclonal Antibody for Flow

CD16 is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by natural killer (NK) cells, and some T cells and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC (antibody dependent cell mediated cytotoxicity) by NK cells. IN comparison, CD32 is a 40 kD glycoprotein that acts as a low affinity receptor for IgG (also known as Fc gamma RII), and mediates several functions including endocytosis, activation of secretion, cytotoxicity and immunomodulation. CD32 is expressed by B cells, monocytes, granulocytes and platelets. Further, CD32 is involved in the phagocytosis of immune complexes, the regulation of antibody production by B-cells, and variations of the CD32 gene may increase vulnerability to systemic lupus erythematosus (SLE). Several transcript variants encoding different isoforms of CD32 have been found.

Specifications

• Antigen: CD16/CD32
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: FRC-4G8
• Conjugate: Unconjugated
• Formulation: PBS with no preservative
• Gene: Fcgr3
• Gene Accession No.: P08101, P08508
• Gene Alias: AI528646; CD16; CD16a; CD16a antigen; CD16B; CD32; CD32 receptor 2; CD32B; CDw32; F630109E10Rik; Fc fragment of IgG low affinity IIIa receptor; Fc fragment of IgG receptor IIb; Fc fragment of IgG receptor IIIa; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity III, receptor for (CD16); Fc fragment of IgG, low affinity IIIa, receptor (CD16a); fc gamma receptor IIB; Fc gamma receptor III; Fc gamma receptor IIIa; Fc gamma receptor III-A; Fc gamma receptor RII; Fc gamma RIIB; Fc gamma RIIIa; Fc receptor, IgG, low affinity IIb; Fc receptor, IgG, low affinity III; Fc[g]RII; Fcg receptor III; FCG2; FCG3; Fc-gamma receptor III-2 (CD 16); Fc-gamma receptor IIIb (CD16); fc-gamma RII; Fc-gamma RII-b; Fc-gamma RII-c; Fc-gamma RIII; Fc-gamma RIIIa; Fc-gamma RIII-alpha; Fc-gamma-RII; FcgammaRIIB; fc-gamma-RIIB; Fc-gamma-RIIc; FcgammaRIII; FcgammaRIIIA; Fcgr2; Fcgr2a; Fcgr2b; FCGR2C; Fcgr3; FCGR3A; FCGR3B; FcgRII; FCGRIII; FCR-10; Fcr-2; Fcr-3; fcRII; FcRII-b; FcRII-c; FCRIII; FCRIIIA; FGFR2B; IGFR3; IgG Fc receptor II beta; IgG Fc receptor III; IgG Fc receptor III-2; IMD20; immunoglobulin G Fc receptor III; low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; low affinity immunoglobulin gamma Fc region receptor III; Low affinity immunoglobulin gamma Fc region receptor III-A; Ly-17; Lym-1; Ly-m20; lymphocyte antigen 17; neutrophil-specific antigen NA; RP11-5K23.1
• Gene Symbols: Fcgr2b, Fcgr3
• Host Species: Rat
• Immunogen: Mouse CD16/32 (Blocks Fc gamma,RIII/II).
• Purification Method: Purified
• Quantity: 2 mL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 14130, 14131
• Target Species: Mouse
• Content And Storage: 4°C
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b

Frequently Asked Questions (FAQs)

What should I use to block my cells for flow cytometry analysis?

Use serum from the same species as the host species of the secondary antibody for blocking. If the serum is not available, use from 2 to 5% BSA (Fraction V, defatted). If using only a primary antibody, such as directly-labeled mouse primary antibodies, a good blocking reagent is Fc block. CD16 + CD32 Antibody (FRC-4G8) (Cat. No. MFCR004 or MA5-16680) is a low-affinity receptor for the Fc region of immunoglobulin gamma complexes.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov