Invitrogen™ CD162 (PSGL-1) Monoclonal Antibody (4RA10), Alexa Fluor™ 647, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 51162180

Catalog No. 51-162-180

Description

Description: This 4RA10 monoclonal antibody reacts with mouse CD162, which is also known as P-selectin glycoprotein ligand-1 (PSGL-1). This clone has been successfully used in flow cytometry and has been reported to block binding of CD162 to CD62P and CD62L. Applications Reported: This 4RA10 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 4RA10 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.06 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser

CD162 (PSGL-1) is a 120 kDa homodimer protein found on the surface of leukocytes and platelets. CD162 is known to bind CD62P (P-selectin), CD62L (L-selectin), and to a lower degree CD62E (E-selectin). These interactions play a critical role in leukocyte adhesion, rolling and extravasation. CD162 has been shown to be an important immune marker of T cell exhaustion in chronic viral infections and cancer.

Specifications

• Antigen: CD162 (PSGL-1)
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q62170
• Gene Symbols: SELPLG
• Immunogen: Mouse PSGL-1 human IgG1 fusion protein.
• Quantity: 25 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG1 κ

• Applications: Flow Cytometry
• Clone: 4RA10
• Conjugate: Alexa Fluor 647
• Gene: SELPLG
• Gene Alias: CD162; CLA; Cutaneous lymphocyte associated antigen; cutaneous lymphocyte-associated associated antigen; leukocyte cell surface adhesion molecule; P-selectin glycoprotein ligand 1; P-selectin glycoprotein ligand 1 propeptide; P-selectin glycoprotein ligand-1; Psgl1; Psgl-1; Selectin P ligand; selectin, platelet (p-selectin) ligand; Selp1; SELPG; Selpl; SELPLG
• Host Species: Rat
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 20345
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

Safety and Handling

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