Invitrogen™ CD163 Monoclonal Antibody (TNKUPJ), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Catalog No.	Quantity
46-163-180	25 μg
46-163-182	100 μg

Catalog No. 46-163-180 Supplier Invitrogen™ Supplier No. 46163180

Description

Rat Monoclonal Antibody

Description: This TNKUPJ monoclonal antibody recognizes mouse CD163. CD163 is a 130kDa surface receptor expressed by certain subsets of tissue macrophages, including splenic red pulp macrophages, Kupffer cells, intestinal lamina propria macrophages and a small fraction of peritoneal macrophages. In contrast to human blood monocytes, mouse monocytes do not express CD163. Also, unlike human CD163, mouse CD163 is not as readily induced by M2 polarizing cytokines, and it is not a good marker of M2 macrophages. No common cell lines of monocytic or macrophage origin express mouse CD163. In humans, CD163 has been shown to be proteolytically cleaved and shed from the cell surface, and it acts as a soluble anti-inflammatory factor. This TNKUPJ antibody will detect CD163 on fixed and permeabilized cells allowing for staining of the intracellular pool of this receptor. Although CD163 is relatively stable to collagenase digestion, aggressive tissue dissociation protocols might potentially decrease the amount of surface CD163. In these cases intracellular detection is recommended. Applications Reported: This TNKUPJ antibody has been reported for use in flow cytometric analysis. Applications Tested: This TNKUPJ antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL.

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein, a member of the scavenger receptor cysteine-rich superfamily, and a receptor for the hemoglobin-haptoglobin complex. CD163 protects tissues from free hemoglobin-mediated oxidative damage, and may play a role in the uptake and recycling of iron, via endocytosis of hemoglobin/haptoglobin and subsequent breakdown of heme. CD163 is expressed exclusively on the cell surface of human monocytes and macrophages that evolve predominantly in the late phase of inflammation. Specifically, CD163 is present on all circulating monocytes and most tissue macrophages except those found in the mantle zone and germinal centers of lymphoid follicles, interdigitating reticulum cells and Langerhan's cells. CD163 is present on all CD14 positive monocytes, most CD64 positive monocytes, and shows higher expression on CD16 positive monocytes. CD163 is upregulated on mononuclear phagocytes by IL-10, IL-6 and dexamethasone. Lipopolysaccharide (LPS) and phorbol myristate acetate (PMA) both induce shedding of CD163 from the cell surface into plasma or cell supernatant. CD163 binds hemoglobin/haptoglobin complexes in a calcium-dependent and pH-dependent manner, and exhibits a higher affinity for complexes of hemoglobin and multimeric haptoglobin of HP1F phenotype than for complexes of hemoglobin and dimeric haptoglobin of HP1S phenotype. Further, CD163 also induces a cascade of intracellular signals that involves tyrosine kinase-dependent calcium mobilization, inositol triphosphate production and secretion of IL6 and CSF1.

Specifications

• Antigen: CD163
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: TNKUPJ
• Concentration: 0.2 mg/mL
• Conjugate: PerCP-eFluor 710
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: CD163
• Gene Accession No.: Q2VLH6
• Gene Alias: CD163; CD163 antigen; CD163 molecule; CD163v2; CD163v3; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; putative CD163 antigen; SCARI1; Scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163; Soluble sCD163
• Gene Symbols: CD163
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 25 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 93671
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.