Invitrogen™ CD170 (Siglec F) Monoclonal Antibody (1RNM44N), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-113-0788	100 μg
50-112-3784	25 μg

Catalog No. 50-113-0788 Supplier Invitrogen™ Supplier No. 46170282

Description

Rat Monoclonal Antibody

Description: This 1RNM44N monoclonal antibody recognizes mouse CD170, also known as Siglec F. Siglec F is a cell surface lectin belonging to the Ig superfamily that binds glycoconjugates containing sialic acids that are commonly found on various cell types. The cytoplasmic domain of Siglec F contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) that initiates intracellular signaling upon ligand binding. Siglec F is expressed mostly on eosinophils and alveolar macrophages and lower levels of this receptor have also been reported on immature myelomonocytic cells. Mouse Siglec F is a functional ortholog of human Siglec 8, however, unlike human Siglec 8, mouse Siglec F is not expressed on mast cells. This 1RNM44N antibody will recognize a formaldehyde-fixed epitope. Applications Reported: This 1RNM44N antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1RNM44N antibody has been tested by flow cytometric analysis of mouse thioglycolate-elicited peritoneal exudate cells. This can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

SIGLEC-F is a sialic acid-binding immunoglobulin-like lectin predominantly expressed on eosinophils in mice, where it serves as a functional paralog of human SIGLEC-8. It acts as an inhibitory receptor with ITIM motifs that recognize sialylated glycans and can induce eosinophil apoptosis, thereby helping regulate allergic inflammation and immune homeostasis.

Specifications

• Antigen: CD170 (Siglec F)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 1RNM44N
• Concentration: 0.2 mg/mL
• Conjugate: PerCP-eFluor 710
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Siglecf
• Gene Accession No.: Q920G3
• Gene Alias: SIGLEC-8
• Gene Symbols: Siglecf
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 233186
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ

Frequently Asked Questions (FAQs)

Can the eFluor Organic fluorochromes be used for intracellular staining?

Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).

What is the difference between eFluor Organic Dyes and eVolve Qdots?

The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.