Invitrogen™ CD200 Monoclonal Antibody (OX90), PE, eBioscience™

Catalog No.	Quantity
50-108-12	100 μg

Catalog No. 50-108-12 Supplier Invitrogen™ Supplier No. 12520082

Description

Rat Monoclonal Antibody

Description: The monoclonal antibody OX90.1 recognizes mouse CD200 also known as OX2. CD200 is a member of the Ig superfamily with 2 Ig domains, a transmembrane and cytoplasmic domain. CD200 is expressed on resting and activated B cells, a subset of resting and activated T cells, keratinocytes, peripheral and central nerve cells, follicular dendritic cells and ovarian cells. The interaction with CD200R results in macrophage activation (IL-6 production), inhibition of mast cell degranulation along with reduced TN Falpha and IL-13 secretion and overall attenuation of the activation status of lymphocytes. Applications Reported: This OX90 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OX90 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD200 is a type-1 membrane glycoprotein in the immunoglobulin superfamily, featuring two immunoglobulin domains. It is widely expressed on various cells, including B and T cells, keratinocytes, nerve cells, follicular dendritic cells, ovarian cells, and trophoblasts. CD200 interacts with CD200R on myeloid cells, inhibiting macrophage activation, reducing IL-6 production, and attenuating lymphocyte activation. It also prevents mast cell degranulation and decreases TNF-alpha and IL-13 secretion, playing a role in immune regulation and maternal tolerance. Homologues of CD200 are found in herpesviruses and poxviruses, indicating a conserved immune modulation function. CD200 has multiple alternatively spliced transcript variants, contributing to its functional diversity. Diseases linked to CD200 dysfunction include Mantle Cell Lymphoma and Primary Hypertrophic Osteoarthropathy, highlighting its importance in health and disease.

Specifications

• Antigen: CD200
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: OX90
• Concentration: 0.2 mg/mL
• Conjugate: PE
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: Cd200
• Gene Accession No.: O54901
• Gene Alias: antigen identified by monoclonal antibody MRC OX-2; CD200; Cd200 antigen; Cd200 molecule; cell surface protein (thymocyte antigen identified by monoclonal antibody MRC-OX2); Cspmo2; hypothetical protein LOC534910; MOX1; MOX2; MRC; MRC OX-2 antigen; MRCOX2; My033; OX2; OX-2; OX-2 membrane glycoprotein
• Gene Symbols: Cd200
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 17470
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2a κ