CD205 Monoclonal Antibody (205yekta), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-151-25	Invitrogen 17205182	100 μg

Description

Description: The 205yekta antibody reacts with mouse CD205, also known as DEC-205 (dendritic and epithelial cells, 205 kDa). CD205 is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 has been shown to bind to PE-Cy5.5 antibody and streptavidin conjugates; therefore, this format should not be used on CD205+ dendritic cells. Applications Reported: This 205yekta antibody has been reported for use in flow cytometric analysis. Applications Tested: This 205yekta antibody has been tested by flow cytometric analysis of in vitro-cultured bone marrow-derived dendritic cells or mouse bone marrow cells. This can be used at less than or equal to 0.03 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD205 (LY25, Lymphocyte Antigen 75) is an integral membrane glycoprotein involved in antigen uptake, trafficking and presentation that improves the induction of antigen-specific T cell immunity. CD205 is highly expressed by CD8+ dendritic cells (DCs) and also expressed at different levels by bone marrow Gr1+ cells, Langerhans cells, (BMDC) bone marrow derived DCs and thymic epithelial cells. CD205 acts as an endocytic receptor that directs captured antigens to the extracellular space towards a specialized antigen processing region. Further, CD205 also prompts the reduced proliferation of B lymphocytes. Diseases associated with CD205 dysfunction include pneumonic plague and lipid pneumonia.

Specifications

• Antigen: CD205
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q60767
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: 205yekta
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 17076
• Primary or Secondary: Primary
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!

For Research Use Only.