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Invitrogen™ CD208 (DC-LAMP) Monoclonal Antibody (31B), APC, eBioscience™
Mouse Monoclonal Antibody
Supplier: Invitrogen™ 17208941

Description
Description: This 31B monoclonal antibody reacts with human CD208, which is also known as dendritic cell lysosome-associated membrane glycoprotein (DC-LAMP). CD208 is expressed almost exclusively by mature dendritic cells. Expression of this molecule can be induced in monocytes by stimulation with GM-CSF and IL-4. Moreover, CD208 can be upregulated in CD34+-derived dendritic cells by GM-CSF, SCF, and TNFa. CD208 is also expressed in dendritic cells of non-lymphoid tissues such as skin. While CD208 can be detected on the cell surface, this protein is mainly localized within lysosomes. CD208 plays a key role in the processing and presentation of antigen during the immune response. The 31B antibody only recognizes intracellular CD208, not the cell surface-expressed protein. Applications Reported: This 31B antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 31B antibody has been pre-titrated and tested by flow cytometric analysis of in vitro differentiated mature dendritic cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.
Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).
Specifications
CD208 (DC-LAMP) | |
Monoclonal | |
5 μL/Test | |
PBS with BSA and 0.09% sodium azide; pH 7.2 | |
Q9UQV4 | |
LAMP3 | |
Affinity chromatography | |
RUO | |
27074 | |
4°C, store in dark, DO NOT FREEZE! | |
Liquid |
Flow Cytometry | |
31B | |
APC | |
LAMP3 | |
1200002D17Rik; CD208; DC LAMP; DCLAMP; DC-LAMP; DC-lysosome-associated membrane glycoprotein; dendritic cells-lysosome associated membrane glycoprotein; LAMP; Lamp3; LAMP-3; lysosomal associated membrane protein 3; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403 | |
Mouse | |
25 Tests | |
Primary | |
Human | |
Antibody | |
IgG2a κ |
Safety and Handling
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