Invitrogen™ CD208 (DC-LAMP) Monoclonal Antibody (31B), APC, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 17208941

Catalog No. 50-245-678

Description

Description: This 31B monoclonal antibody reacts with human CD208, which is also known as dendritic cell lysosome-associated membrane glycoprotein (DC-LAMP). CD208 is expressed almost exclusively by mature dendritic cells. Expression of this molecule can be induced in monocytes by stimulation with GM-CSF and IL-4. Moreover, CD208 can be upregulated in CD34+-derived dendritic cells by GM-CSF, SCF, and TNFa. CD208 is also expressed in dendritic cells of non-lymphoid tissues such as skin. While CD208 can be detected on the cell surface, this protein is mainly localized within lysosomes. CD208 plays a key role in the processing and presentation of antigen during the immune response. The 31B antibody only recognizes intracellular CD208, not the cell surface-expressed protein. Applications Reported: This 31B antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This 31B antibody has been pre-titrated and tested by flow cytometric analysis of in vitro differentiated mature dendritic cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

Dendritic cells (DCs) are the most potent antigen-presenting cells. Immature DCs efficiently capture antigens and differentiate into interdigitating dendritic cells (IDCs) in lymphoid tissues that induce primary T-cell responses (summary by de Saint-Vis et al., 1998 [PubMed 9768752]).

Specifications

• Antigen: CD208 (DC-LAMP)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9UQV4
• Gene Symbols: LAMP3
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 27074
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: 31B
• Conjugate: APC
• Gene: LAMP3
• Gene Alias: 1200002D17Rik; CD208; DC LAMP; DCLAMP; DC-LAMP; DC-lysosome-associated membrane glycoprotein; dendritic cells-lysosome associated membrane glycoprotein; LAMP; Lamp3; LAMP-3; lysosomal associated membrane protein 3; lysosomal-associated membrane protein 3; lysosome-associated membrane glycoprotein 3; Protein TSC403; TSC403
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

Safety and Handling

WARNING: Cancer - www.P65Warnings.ca.gov