Invitrogen™ CD215 (IL-15Ra) Monoclonal Antibody (DNT15Ra), PerCP-eFluor™ 710, eBioscience™

Catalog No.	Quantity
46-714-982	100 μg

Catalog No. 46-714-982 Supplier Invitrogen™ Supplier No. 46714982

Description

Rat Monoclonal Antibody

This DNT15Ra antibody reacts with the alpha subunit of the Mouse IL-15 Receptor. The IL-15 Receptor alpha subunit is unique to IL-15, while the other two subunits of the receptor, beta and gamma, are shared with IL-2. IL-15R signaling is mediated by Jak/STAT pathways, and results in the activation of neutrophils, dendritic cells, and macrophages. IL-15 has also been found to play a critical role in the development and survival of NK cells and CD8+ T cells. Although high levels of mouse IL-15 mRNA have been observed in many cell types, free IL-15 is rarely detectable in biological samples. Recent research suggests that IL-15 is retained inside the cell and only secreted in complex with IL-15 Receptor alpha. This chaperoning is necessary from the ER to the Golgi, through secretion. The cytokine/receptor complex may stay bound to the cell surface, where it can be trans-presented to cells expressing the beta-gamma subunits of the receptor, or can be secreted in a soluble form lacking the transmembrane domain of IL-15 Receptor alpha. IL-15 and IL-15 Receptor alpha are expressed by dendritic cells and macrophages in response to inflammatory stimuli. Our testing suggests that there may be strain differences in expression and staining of the Mouse IL-15 Receptor alpha. This DNT15Ra antibody has been tested on cells from C57Bl/6 mice. Staining has also been observed with this antibody after pre-incubation of NK cells with exogenous Mouse IL-15/IL-15R Complex, suggestin...

Interleukin 15 (IL-15RA) is a cytokine receptor that specifically binds IL-15 with high affinity. IL-15 regulates T and natural killer cell activation and proliferation. The IL-15 and IL-2 receptors share two subunits, IL-2R beta and IL-2R gamma, and IL-15RA is structurally related to IL-2RA, an additional IL-2-specific alpha subunit necessary for high affinity IL-2 binding. Unlike IL-2RA, IL-15RA is capable of binding IL-15 with high affinity independent of the other subunits. IL-15RA is thought to enhance cell proliferation and expression of apoptosis inhibitor Bcl-xL and Bcl-2.

Specifications

• Antigen: CD215 (IL-15Ra)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: DNT15Ra
• Concentration: 0.2 mg/mL
• Conjugate: PerCP-eFluor 710
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: IL15RA
• Gene Accession No.: Q60819
• Gene Alias: AA690181; CD215; IL 15 receptor; IL 15 receptor subunit α; IL 15R; IL 15R alpha; IL 15R α; IL15 receptor; IL15 receptor subunit alpha; IL-15 receptor subunit alpha; IL15 receptor subunit α; IL15R; IL15R alpha; IL15R α; Il15ra; IL-15RA; IL-15R-alpha; Interleukin 15 receptor; interleukin 15 receptor alpha isoform EM2; interleukin 15 receptor alpha isoform IC2; interleukin 15 receptor alpha isoform IC3; interleukin 15 receptor alpha isoform IC4; interleukin 15 receptor alpha isoform IC5; interleukin 15 receptor alpha isoform IC6; interleukin 15 receptor alpha isoform IC7; interleukin 15 receptor alpha isoform IC8; interleukin 15 receptor subunit alpha; Interleukin 15 receptor subunit α; interleukin 15 receptor, alpha; interleukin 15 receptor, alpha chain; interleukin-15 receptor subunit alpha; sIL 15R; sIL-15 receptor subunit alpha; sIL15R; sIL-15RA; sIL-15R-alpha; soluble IL 15R; soluble IL15R; Soluble interleukin-15 receptor subunit alpha
• Gene Symbols: IL15RA
• Host Species: Rat
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 16169
• Target Species: Mouse
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1 κ

Frequently Asked Questions (FAQs)

I am unable to analyze my cells stained with eFluor Organic Dyes today. What options do I have?

Our options will depend on the samples you are analyzing.

If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.

For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.

What buffers are compatible with the eFluor Organic fluorochromes and eVolve QDots?

The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.

Are the eFluor Organic Dyes photo-labile?

As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.