CD223 (LAG-3) Monoclonal Antibody (3DS223H), Brilliant Violet™ 421, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
40-422-3941	Invitrogen™ 404223941	25 Tests
40-422-3942	Invitrogen™ 404223942	100 Tests

Description

This 3DS223H mAb recognizes human CD223 also known as Lymphocyte Activation Gene 3 (LAG-3). LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells). This 3DS223H antibody will recognize a formaldehyde-fixed epitope. Applications Tested: This 3DS223H antibody has been pre-diluted and tested by flow cytometric analysis of stimulated normal human peripheral blood cells. BV 421 is a dye that emits at 423nm and is intended for use on cytometers equipped with a violet (405nm) laser. When using two or more Super Bright, BV, BUV, or other polymer dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (SB-4401-42) or Brilliant Stain Buffer (00-4409-75) to minimize any non-specific polymer interactions. Excitation: 407nm; Emission: 423nm; Laser: Violet Laser. BV is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen.

LAG-3 is a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds to MHC class II with higher affinity than CD4 and is thought to be involved in the negative regulation of T cell activation and homeostatic proliferation. Surface expression of LAG-3 has been reported on activated T cells (including regulatory T cells) and NK cells. CD8+ T cells usually express LAG-3 at significantly higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b has been proposed to identify human and mouse Type 1 regulatory T cells (Tr1 cells).

Specifications

• Antigen: CD223 (LAG-3)
• Concentration: 5 μL/Test
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Alias: Activation-induced cytidine deaminase-linked autoimmunity protein; Aida; CD223; FDC; LAG3; LAG-3; Ly66; lymphocyte activating 3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Secreted lymphocyte activation gene 3 protein; sLAG 3; sLAG3; sLAG-3; soluble LAG 3lymphocyte activating 3; soluble LAG3
• Gene Symbols: LAG3
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: LAG3

• Clone: 3DS223H
• Applications: Flow Cytometry
• Conjugate: Brilliant Violet 421
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: P18627
• Gene ID (Entrez): 3902
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody