Invitrogen™ CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), FITC, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-112-9388	100 μg
50-101-17	25 μg

Catalog No. 50-112-9388 Supplier Invitrogen™ Supplier No. 11998782

Description

Mouse Monoclonal Antibody

Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells. Applications Reported: The HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis. Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at less than or equal to 0.03 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Glycophorin A, also known as CD235a, is a 151 amino acid sialoglycoprotein expressed on the membrane of mature erythrocytes and erythroid progenitor cells, with approximately 500,000 copies per cell. The gene for glycophorin A is located on chromosome 4 and exists in two allelic forms, M and N, which differ by two amino acids: the M group has Ser1 and Gly5, while the N group has Leu1 and Glu5. These allelic variations define the blood group M and N specificities. Glycophorin A serves multiple functions, including providing a mucin-like barrier that minimizes aggregation between red blood cells in circulation, potentially preventing cell fusion. It also acts as a receptor for certain pathogens, including Sandei virus, parvovirus, and Hsa, a Streptococcus adhesin. Recent studies suggest that exposure to toxins can lead to mutations or loss of alleles, resulting in phenotypic changes. There is ongoing research correlating genotype and phenotype with predisposition to diseases such as cancer and heart disease, highlighting the importance of glycophorin A in both health and disease. Glycophorin A is a significant marker for studying erythrocyte-related functions and pathologies, as well as its role in pathogen interactions and disease predisposition.

Specifications

• Antigen: CD235a (Glycophorin A)
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: HIR2 (GA-R2)
• Concentration: 0.5 mg/mL
• Conjugate: FITC
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene: GYPA
• Gene Accession No.: P02724
• Gene Alias: AI853584; CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A; glycophorin A (MN blood group); glycophorin A (MNS blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; glycophorin-A; GPA; GPErik; GPSAT; Gypa; HGNC:4702; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN; MN sialoglycoprotein; MNS; PAS-2; recombinant glycophorin A-B Miltenberger-DR; sialoglycoprotein alpha
• Gene Symbols: GYPA
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 μg
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 2993
• Target Species: Human
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.