Invitrogen™ CD244.1 Monoclonal Antibody (C9.1), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Supplier:  Invitrogen™ 17244082

Catalog No. 17-244-082

Description

Description: The monoclonal antibody C9.1 reacts with mouse CD244.1. This receptor is expressed on NK cells, gamma delta T cells, some CD8+ T cells, basophils, and monocytes on most inbred mouse strains except C57BL/6 and C58, which express the CD244.2 allotype. CD244 is a CD2-related receptor belonging to the SLAM family that exists as two different isoforms with opposing functions. CD244 associates with CD48, which is expressed widely on hematopoietic cells, regulates NK cell proliferation, cytotoxicity, and cytokine production. However, the CD244/CD28 interaction between NK and other cell types inhibits NK cytotoxicity, which contributes to self-tolerance. CD244 may enhance or inhibit CD8+ T cell effector function depending on SAP levels. The C9.1 antibody has been reported to have antagonist activity on activated NK cells. Applications Reported: This C9.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This C9.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Specifications

• Antigen: CD244.1
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q07763
• Gene Symbols: Cd244
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 18106
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: C9.1
• Conjugate: APC
• Gene: Cd244
• Gene Alias: 2B4; C9.1; CD244; CD244 long isoform; CD244 natural killer cell receptor 2B4; CD244 short isoform; F730046O15Rik; Ly90; NAIL; natural killer cell receptor; Natural killer cell receptor 2B4; NK cell receptor 2B4; NK cell type I receptor protein 2B4; NKR2B4; Nmrk; non MHC restricted killing associated; non-MHC restricted killing associated; Signaling lymphocytic activation molecule 4; SLAM family member 4; SLAMF4
• Host Species: Rat
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2b κ

For Research Use Only.