CD244.1 Monoclonal Antibody (C9.1), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
17-244-082	Invitrogen™ 17244082	100 μg

Description

Description: The monoclonal antibody C9.1 reacts with mouse CD244.1. This receptor is expressed on NK cells, gamma delta T cells, some CD8+ T cells, basophils, and monocytes on most inbred mouse strains except C57BL/6 and C58, which express the CD244.2 allotype. CD244 is a CD2-related receptor belonging to the SLAM family that exists as two different isoforms with opposing functions. CD244 associates with CD48, which is expressed widely on hematopoietic cells, regulates NK cell proliferation, cytotoxicity, and cytokine production. However, the CD244/CD28 interaction between NK and other cell types inhibits NK cytotoxicity, which contributes to self-tolerance. CD244 may enhance or inhibit CD8+ T cell effector function depending on SAP levels. The C9.1 antibody has been reported to have antagonist activity on activated NK cells. Applications Reported: This C9.1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This C9.1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

CD244.1 Monoclonal antibody specifically detects CD244.1 in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD244.1
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: Q07763
• Gene Symbols: Cd244
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Gene: Cd244

• Clone: C9.1
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 18106
• Isotype: IgG2b κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

For Research Use Only.