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CD244.2 (2B4) Rat anti-Mouse, Clone: eBio244F4, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$75.25 - $269.00

Specifications

Antigen CD244.2 (2B4)
Clone eBio244F4
Target Species Mouse
Conjugate Unconjugated
Isotype IgG2a
View More Specs

Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-129-98 Non-distribution product as customer accommodation.
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Invitrogen
14244182
100 μg
Each for $117.00
Add to cart
 
50-129-97 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14244181
50 μg
Each for $75.25
Add to cart
 
50-129-99 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14244185
500 μg
Each for $269.00
Add to cart
 
Description

Description

Description: Mouse CD244, also known as 2B4, is a receptor belonging to the CD2 family of proteins involved in non-MHC dependent cytotoxicity. Expression is found on natural killer (NK) cells and many NKT cells and CD8+ cells. In C57BL/6 mice, two splice variants exist that differ in the length of the cytoplasmic domain. The long form functions in an inhibitory manner through signaling through SHP-2, while the short form functions in an activating manner. The ligand for CD244 is CD48 which is expressed on all hematopoietic cells. Recent data suggests that CD244 interactions with CD48, but not CD2 interaction with CD48, is essential for IL-2-driven expansion and activation of murine NK cells. Reports show CD244 expression can discriminate hematopoietic progenitors. Hematopoietic stem cells (HSC) are highly purified as CD150+CD244-CD48- cells while non-self-renewing multipotent hematopoietic progenitors (MPP) are CD244+CD150-CD48- and the most restricted progenitors are CD48+CD244+CD150-. eBio244F4 was generated against cell lines expressing CD244. It reacts with the C57BL/6 but not Balb/c due to polymorphisms present in the immunoglobulin V domain of the protein. The epitope recognized by eBio244F4 is different from that of 2B4; therefore these antibodies do not cross-block the binding to the respective epitopes. Applications Reported: This eBio244F4 antibody has been reported for use in flow cytometric analysis and immunoblotting (WB).

Applications Tested: This eBio244F4 antibody has been tested by flow cytometric analysis of IL-2-stimulated mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. This gene encodes a cell surface receptor expressed on natural killer (NK) cells (and some T cells) that mediate non-major histocompatibility complex (MHC) restricted killing. The interaction between NK-cell and target cells via this receptor is thought to modulate NK-cell cytolytic activity. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Specifications

Specifications

CD244.2 (2B4)
Mouse
IgG2a
Monoclonal
Liquid
Antibody
4° C
eBio244F4
Unconjugated
Flow Cytometry, Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

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