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Invitrogen™ CD249 (BP-1) Monoclonal Antibody (6C3), PerCP-eFluor™ 710, eBioscience™
Description
The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7. Applications Reported: This 6C3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 1 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. PerCP-eFluor 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please p...
Specifications
Specifications
| Antigen | CD249 (BP-1) |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | 6C3 |
| Concentration | 0.2 mg/mL |
| Conjugate | PerCP-eFluor 710 |
| Formulation | PBS with 0.09% sodium azide; pH 7.2 |
| Gene | Enpep |
| Gene Accession No. | P16406 |
| Gene Alias | 6030431M22Rik; Aminopeptidase A; aminopeptidase-A; APA; AP-A; BP1; Bp-1/6C3; BP-1/6C3 antigen; CD249; differentiation antigen gp160; EAP; ENPEP; Glutamyl aminopeptidase; glutamyl aminopeptidase (aminopeptidase A); gp160; Ly51; Ly-51 |
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Frequently Asked Questions (FAQs)
Our options will depend on the samples you are analyzing.
If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.
For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.
Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).
The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.
The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.
As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.
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