CD253 (TRAIL) Monoclonal Antibody (N2B2), APC, eBioscience™, Invitrogen™

Rat Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-112-3169	Invitrogen™ 17595182	100 μg

Description

Description: The N2B2 monoclonal antibody reacts with mouse TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is not detected on the surface of freshly isolated T, B, or NK cells, but can be induced preferentially on CD3- NK1.1+ NK cells after stimulation with IL-2 or IL-15. N2B2 inhibits IL-2- or IL-15-activated NK cell cytotoxicity against mouse fibrosarcoma L929 target cells. Applications Reported: This N2B2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This N2B2 antibody has been tested by flow cytometric analysis of mouse CD253 transfected cells and can be used at less than or equal to 0.125 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD253 (TRAIL) Monoclonal antibody specifically detects CD253 (TRAIL) in Mouse samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD253 (TRAIL)
• Concentration: 0.2 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Gene Accession No.: P50592
• Isotype: IgG2a κ
• Purification Method: Affinity chromatography
• Product Type: Antibody

• Clone: N2B2
• Applications: Flow Cytometry
• Conjugate: APC
• Host Species: Rat
• Target Species: Mouse
• Gene ID (Entrez): 22035
• Primary or Secondary: Primary
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!

For Research Use Only.