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CD274 (PD-L1, B7-H1) Monoclonal Antibody (MIH1), APC-eFluor™ 780, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 47598342

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Catalog No. 47598342


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Description

Description

Description: The MIH1 monoclonal antibody reacts with human B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH1 is reported to be a blocking antibody. Applications Reported: This MIH1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This MIH1 antibody has been pre-titrated and tested by flow cytometric analysis of stimulated human peripheral blood cells. This can be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. APC-eFluor™ 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochrome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product No.

CD274 (PD-L1, B7-H1) Monoclonal antibody specifically detects CD274 (PD-L1, B7-H1) in Human samples. It is validated for Flow Cytometry.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

CD274 (PD-L1, B7-H1)
Monoclonal
5 μL/Test
PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
Q9NZQ7
Cd274
Affinity chromatography
RUO
29126
4° C, store in dark, DO NOT FREEZE!
Liquid
Flow Cytometry
MIH1
APC-eFluor 780
Cd274
A530045L16Rik; B7 homolog 1; B7-H; B7h1; B7-H1; Cd274; CD274 antigen; CD274 molecule; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; PD-L1; pdl-1; Programmed cell death 1 ligand 1; programmed death ligand 1; RGD1566211; sCD274; soluble CD274; soluble PD L1; sPD L1
Mouse
100 Tests
Primary
Human
Antibody
IgG1 κ
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