Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

CD274 (PD-L1, B7-H1) Rat anti-Mouse, Clone: MIH5, eBioscience™ Non-distribution product as customer accommodation.

Rat Monoclonal Antibody

$72.75 - $305.00

Specifications

Antigen CD274 (PD-L1, B7-H1)
Clone MIH5
Target Species Mouse
Conjugate Unconjugated
Isotype IgG2a, lambda
View More Specs

Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
50-112-8872 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14598281
50 μg
Each for $72.75
Add to cart
 
50-112-2704 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14598282
100 μg
Each for $119.00
Add to cart
 
50-134-02 Non-distribution product as customer accommodation.
View Documents
Invitrogen
14598285
500 μg
Each for $305.00
Add to cart
 
Description

Description

Description: The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody. Applications Reported: The MIH5 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections. It has also been reported in blocking in in vitro and in vivo functional assays. (Please use Functional Grade Purified MIH5, cat. 16-5982, in functional assays. Fluorochrome-conjugated MIH5 is recommended for use in flow cytometry.). Applications Tested: This MIH5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered. Programmed death receptor ligand 1 (PD-L1, also called B7-H1) is a recently described B7 family member. To date, one specific receptor has been identified that can be ligated by PD-L1. This receptor, programmed death receptor 1 (PD-1), has been shown to negatively regulate T-cell receptor (TCR) signaling. Upon ligating its receptor, PD-L1 has been reported to decrease TCR-mediated proliferation and cytokine production. PD-L1 expression was found to be abundant on many murine and human cancers and could be further up-regulated upon IFN-gamma stimulation. Thus, PD-L1 might play an important role in tumor immune evasion.
Specifications

Specifications

CD274 (PD-L1, B7-H1)
Mouse
IgG2a, lambda
Monoclonal
Liquid
Antibody
4° C
MIH5
Unconjugated
Flow Cytometry, Functional Assay, Immunohistochemistry (Frozen), Neutralization, Western Blot
Primary
Monoclonal
PBS with 0.09% sodium azide; pH 7.2
RUO
SDS

For Research Use Only.

Provide Content Correction

The Fisher Scientific Encompass Program offers items which are not part of our distribution portfolio. These products typically do not have pictures or detailed descriptions. However, we are committed to improving your shopping experience. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit