CD279 (PD-1) Monoclonal Antibody (eBioJ105 (J105)), PerCP-eFluor™ 710, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  Invitrogen 46279941

Catalog No. 50-112-3798

Description

Description: The J105 monoclonal antibody reacts with the human PD-1 (programmed death-1), a 55 kDa member of the CD28 immunoglobulin superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease. PD-1 is expressed predomitly on activated T and B lymphocytes. Two novel members of the B7 family have been identified as the PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these two PD-1 ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. Costaining experiments suggest that eBioJ105 recognizes a different epitope than MIH4 (cat. 11-9969). Applications Reported: This eBioJ105 (J105) antibody has been reported for use in flow cytometric analysis. Applications Tested: This eBioJ105 (J105) antibody has been pre-titrated and tested by flow cytometric analysis of activated human peripheral blood cells. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor™ 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor™ 710 emits at 710 nm and is excited with the blue laser (488 nm).

CD279 (PD-1) Monoclonal antibody specifically detects CD279 (PD-1) in Human, Rhesus Monkey samples. It is validated for Flow Cytometry.

Specifications

• Antigen: CD279 (PD-1)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q15116
• Gene Symbols: Pdcd1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 100135775, 5133
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: eBioJ105 (J105)
• Conjugate: PerCP-eFluor 710
• Gene: Pdcd1
• Gene Alias: CD279; EGK_05005; hPD1; hPD-1; hPD-l; hSLE1; Ly101; mPD-1; PD1; PD-1; Pdc1; Pdcd1; programmed cell death 1; programmed cell death 1 protein; programmed cell death protein 1; programmed cell death protein 1-like; programmed death 1; Protein PD1; protein PD-1; sCD279; SLEB2; soluble CD279; systemic lupus erythematosus susceptibility 2
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human, Rhesus Monkey
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.