Invitrogen™ CD282 (TLR2) Monoclonal Antibody (TL2.1), Functional Grade, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 16992282

Catalog No. 50-112-3015

Description

Description: The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC. Applications Reported: TL2.1 has been reported for use in flow cytometric analysis and blocking of TLR2-mediated cytokine production. Inhibition of cytokine production has been reported in the literature using Listeria monocytogenes and peptidoglycans. Optimal conditions for the inhibition assays using TL2.1 and specific stimulatory reagents/suppliers have to be evaluated by individual investigators. Neutralization Protocol: (Important note: make sure to eliminate LPS contamination in the culture system) 1) Prepare target cells of in...

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

Specifications

• Antigen: CD282 (TLR2)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2
• Gene Accession No.: O60603
• Gene Symbols: Tlr2
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 7097
• Content And Storage: 4°C
• Form: Liquid

• Applications: Flow Cytometry, Functional Assay, Neutralization
• Clone: TL2.1
• Conjugate: Functional Grade
• Gene: Tlr2
• Gene Alias: CD282; Ly105; TIL4; Tlr2; TLR-2; toll like receptor 2; toll/interleukin-1 receptor-like protein 4; Toll-like receptor; toll-like receptor 2; toll-like receptor 2 variant 1; toll-like receptor 2 variant 2; toll-like-receptor 2, exon 1a_; Toll-like-receptor, exon 1b; Toll-like-receptor, exon 1b_; Toll-like-receptor2, exon 1a
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov