Invitrogen™ CD298 Monoclonal Antibody (M016)

Catalog No.	Quantity
PIMA547672	100 μL

Catalog No. PIMA547672

Supplier: Invitrogen™ MA547672

Description

Mouse Monoclonal Antibody

Clone M016 mouse monoclonal antibody detects a 40 kDa* protein on SDS-PAGE Native immunoblots of human A431, LNCaP, MeWo, MDA-MB-231, and MCF7 cells. This antibody does not detect denatured Na+/K+ ATPase beta3 subunit. In addition, mass spectrometry analysis of immunoprecipitates using NM0161 in human A431 cell lysates confirms that this antibody only detects Na+/K+ ATPase beta3 protein.The antibody works for western blot, immunoprecipitation, ELISA, and immunocytochemistry, as well as detects the beta3 subunit on live cells.

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2.

Specifications

• Antigen: CD298
• Applications: Immunoprecipitation, Western Blot, Immunocytochemistry
• Classification: Monoclonal
• Clone: M016
• Concentration: 0.5 mg/mL
• Conjugate: Unconjugated
• Formulation: PBS with 1mg/mL BSA, 50% glycerol and 0.05% sodium azide
• Gene: ATP1B3
• Gene Accession No.: P54709
• Gene Alias: AA409958; AI664000; Atp1b3; ATPase Na+/K+ transporting subunit beta 3; ATPase, Na+/K+ beta 3 polypeptide; ATPase, Na+/K+ transporting, beta 3 polypeptide; ATPase, Na+K+ transporting, beta polypeptide 3; ATPB-3; AW212096; CD298; CD298 antigen; Na K-ATPase beta-3 subunit; Na+/K+ -ATPase beta 3 subunit; NKAB3S; Sodium Potassium ATPase; Sodium/potassium-dependent ATPase subunit beta-3; sodium/potassium-transporting ATPase subunit beta-3
• Gene Symbols: ATP1B3
• Host Species: Mouse
• Immunogen: Clone (M016) was generated from a proprietary antigen related to the native human Na+/K+ ATPase beta3 subunit expressed in A431 epidermoid carcinoma cell line.
• Purification Method: Protein G
• Quantity: 100 μL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 483
• Target Species: Human
• Content And Storage: -20°C, Avoid Freeze/Thaw Cycles
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1