CD3 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: UCHT1, BD Horizon™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
BDB612751	BD Biosciences 612751	25 Tests
BDB612750	BD Biosciences 612750	100 Tests

Description

The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .

The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).

Specifications

• Antigen: CD3
• Applications: Flow Cytometry
• Conjugate: Brilliant Ultraviolet 737
• Regulatory Status: RUO
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Isotype: IgG1 κ
• Purification Method: Affinity Purified

• Clone: UCHT1
• Classification: Monoclonal
• Host Species: Mouse
• Target Species: Human
• Immunogen: Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
• Primary or Secondary: Primary
• Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.