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  6. CD3 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: UCHT1, BD Horizon™

CD3 Mouse anti-Human, Brilliant Ultraviolet 737, Clone: UCHT1, BD Horizon™

Catalog No. p-7161707 Shop All BD Biosciences Products
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BDB612751 25 Tests
BDB612750 100 Tests
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Description

Mouse Monoclonal Antibody

The UCHT1 monoclonal antibody specifically binds to the human CD3 ε -chain, a 20-kDa subunit of the CD3/T cell antigen receptor complex. CD3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the HLDA Workshop show that this antibody is mitogenic for CD3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-CD28 antibody. CD3 plays a central role in signal transduction during antigen recognition. The UCHT1 antibody stains both surface and intracellular CD3 ε unlike the other CD3 clone, HIT3a, that stains only extracellular CD3 ε .

The antibody was conjugated to BD Horizon™ BUV737 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes. This dye is a tandem fluorochrome of BD Horizon BUV395 with an Ex Max of 348-nm and an acceptor dye with an Em Max at 737-nm. BD Horizon Brilliant BUV737 can be excited by the ultraviolet laser (355 nm) and detected with a 740/35 filter. Due to the excitation of the acceptor dye by other laser lines, there may be significant spillover into channels detecting Alexa Fluor™ 700-like dyes (e.g., 712/20-nm filter).

TRUSTED_SUSTAINABILITY

Specifications

Antigen CD3
Applications Flow Cytometry
Classification Monoclonal
Clone UCHT1
Conjugate Brilliant Ultraviolet 737
Formulation Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species Mouse
Immunogen Human infant thymocytes and peripheral blood lymphocytes from a Sézary Syndrome donor
Purification Method Affinity Purified
Quantity 25 Tests
Regulatory Status RUO
Primary or Secondary Primary
Target Species Human
Content And Storage Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Isotype IgG1 κ
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Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov
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