Learn More
Invitrogen™ CD300f (IREM-1) Monoclonal Antibody (UP-D1), eFluor™ 660, eBioscience™
Description
Description: The monoclonal antibody recognizes human IREM-1 (Immune receptor expressed by myeloid cell 1). IREM-1 is also a member of of the CD300 family specifically referred to as CD300f. IREM-1 is a type I transmembrane protein with a single Ig domain, one predicted N-linked glycosylation site and a long cytoplasmic tail which contains a predicted ITIM domain categorizing it as an inhibitory receptor. Additionally the cytoplasmic domain has been shown to interact with SHP-1 and the p85\alpha subunit of PI3K. Expression is restricted to myeloid cells. In peripheral blood IREM-1 is found on monocytes and granulocytes. In monocyte derived dendritic cells, expression of IREM-1 is downregulated. Applications Reported: This UP-D1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UP-D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 ...
Specifications
Specifications
| Antigen | CD300f (IREM-1) |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | UP-D1 |
| Concentration | 5 μL/Test |
| Conjugate | eFluor 660 |
| Formulation | PBS with BSA and 0.09% sodium azide; pH 7.2 |
| Gene | CD300LF |
| Gene Accession No. | Q8TDQ1 |
| Gene Alias | CD300 antigen like family member F; CD300 antigen-like family member F; CD300 molecule like family member f; CD300 molecule-like family member f; CD300f; Cd300lf; CLIM1; CLM1; CLM-1; CMRF-35-like molecule 1; CMRF35-like molecule 1; DC-derived Ig-like receptor 2; Digr2; F730004D16Rik; IgSF13; Immune receptor expressed on myeloid cells 1; immunoglobin superfamily member 13; immunoglobulin superfamily member 13; inhibitory receptor IREM1; IREM1; IREM-1; Leukocyte mono-Ig-like receptor 3; Lmir3; MAIR-5; MAIR-V; myeloid-associated immunoglobulin-like receptor 5; NK inhibitory receptor; NKIR; Pigr3; UNQ3105/PRO10111 |
| Show More |
Frequently Asked Questions (FAQs)
Our options will depend on the samples you are analyzing.
If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.
For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.
Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).
Yes, in-house studies have demonstrated that the eFluor 660 fluorochrome is recognized by Anti-Cy5/Alexa Fluor 647 beads. Side by side studies with Alexa Fluor 647 versus eFluor 660 conjugated antibodies have demonstrated comparable results.
The eFluor Organic fluorochromes and eVolve QDots can be used with flow staining buffers containing PBS and protein.
The eFluor Organic Dyes (eFluor 450, APC-eFluor 780, PerCP-eFluor 710, eFluor 710) are conventional fluorochromes. In contrast, the eVolve line of products are Quantum dots.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.