Novus Biologicals™ CD31/PECAM-1 Antibody Pair [HRP]

Description

Mouse Monoclonal Antibody Pair

This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD31/PECAM-1.

Specifications

• Assay Range: 18.75 pg/mL
• Assay Sensitivity: 18.75-12000 pg/mL
• Assay: Sandwich ELISA
• Conjugate: HRP
• Product Type: Antibody Pairs
• Specificity: This solid phase sandwich ELISA utilizes a monoclonal antibody specific for Human CD31/PECAM-1.
• Target Species: Human
• For Use With (Application): Sandwich ELISA
• Gene Alias: adhesion molecule, CD31, CD31 antigen, CD31/EndoCAM, EndoCAM, FLJ34100, FLJ58394, GPIIA', PECA1, PECAM-1, PECAM-1, CD31/EndoCAM, platelet endothelial cell adhesion molecule, platelet/endothelial cell adhesion molecule
• Gene ID (Entrez): 5175
• Gene Symbol: PECAM1
• Host Species: Mouse
• Kit Contents:
  Detection Antibody: 0.2 mg/mL of mouse anti-Human CD31/PECAM-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4C).
  Capture Antibody: 1 mg/mL of mouse anti-Human CD31/PECAM-1 monoclonal antibody (in PBS, pH 7.4).
  Standard: Each vial contains 615 ng of recombinant Human CD31/PECAM-1.
• Quantity: 15 Plates
• Regulatory Status: RUO
• Research Discipline: Angiogenesis, Cancer, Cell Biology, Cellular Markers, Endothelial Cell Markers, Extracellular Matrix, Hematopoietic Stem Cell Markers, Immunology, Mesenchymal Stem Cell Markers, Myeloid Cell Markers, Myeloid derived Suppressor Cell, Signal Transduction, Stem Cell Markers
• Storage Requirements: Storage is content dependent.
• Target: CD31/PECAM-1
• Technique: 1. Capture Antibody: Dilute to a working concentration of 2 μg/mL in PBS before coating. 2. Detection Antibody: Dilute to working concentration of 0.8 μg/mL in detection antibody dilution buffer before use. 3. Standard: Reconstitute with 1 ml detection antibody dilution buffer. After reconstitution, store at -20 to -80°C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 12000 pg/mL is recommended.