Invitrogen™ CD31 (PECAM-1) Monoclonal Antibody (Gi-34), eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ BMS1020

Catalog No. 50-176-83

Description

Description: Recognizes the Ig loops 2-3 of human CD31. PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation. PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines. Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction. PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis. Several data have suggested that PECAM-1 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation. Applications Tested: ELISA, Flow Cytometry, Immunoprecipitation, Western Blotting, Monoclonal antibody immobilization of platelet antigens (MAIPA).

CD31 (platelet endothelial cell adhesion molecule-1, PECAM-1) is an inhibitory coreceptor involved in regulation of T cell and B cell signaling by a dual immunoreceptor tyrosine-based inhibitory motif (ITIM) that upon associated kinases-mediated phosphorylation provide docking sites for protein-tyrosine phosphatases. CD31 is expressed ubiquitously within the vascular compartment and is located mainly at junctions between adjacent cells. N-terminal Ig-like domain of CD31 is responsible for its homophilic binding, which plays an important role in cell-cell interactions. CD31 is a multifunctional molecule with diverse roles in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, negative regulation of immunoreceptor signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. It is one of key regulatory molecules in vascular system.

Specifications

• Antigen: CD31 (PECAM-1)
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with no preservative; pH 7.2-7.4
• Gene Accession No.: P16284
• Gene Symbols: Pecam1
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 5175
• Content And Storage: -20°C
• Form: Liquid

• Applications: ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: Gi-34
• Conjugate: Unconjugated
• Gene: Pecam1
• Gene Alias: C85791; CD31; CD31 antigen; CD31, PECAM-1; CD31/EndoCAM; endoCAM; GPIIA'; I79_008304; PECA1; Pecam; PECAM1; Pecam-1; platelet and endothelial cell adhesion molecule 1; platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule-1; platelet/endothelial cell adhesion molecule (CD31 antigen); platelet/endothelial cell adhesion molecule 1; type I transmembrane endothelial adhesion molecule
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov