CD321 (F11R) Mouse anti-Human, PE, Clone: CSTEM27, eBioscience™ 

Mouse Monoclonal Antibody

Manufacturer:  Invitrogen 12321942

Catalog No. 12321942

Description

This monoclonal antibody CSTEM27 reacts with human CD321 (F11R, JAM-1, JAM-A). Applications Reported: This CSTEM27 antibody has been reported for use in flow cytometric analysis. Applications Tested: This CSTEM27 antibody has been pre-diluted and tested by flow cytometric analysis of human iPSCs. This may be used at 5 μL (0.125 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD321 (F11R, JAM-1, JAM-A) is a cell-surface adhesion molecule belonging to the Ig superfamily of receptors. CD321 is a 32-41 kD glycoprotein, possessing two extracellular Ig-like domains, a transmembrane region, and a short cytosolic tail. The protein assembles at the membrane as a homodimer, and can associate laterally with other membrane proteins including integrins. CD321 is expressed on a variety of cell types, including leukocytes, platelets, erythrocytes, stem cells, epithelial and endothelial cells, and plays a role in mediating cell-cell contact. CD321 can be found in close proximity to several components of epithelial tight junctions, including ZO-1 and occludin. CD321 is involved in the maintece of epithelial and endothelial barriers, hematopoiesis, angiogenesis, and tumor proliferation, invasion, and metastasis.

Specifications

• Antigen: CD321 (F11R)
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: Q9Y624
• Gene Alias: F11 R, JAMA, junctional adhesion molecule A, JAM-1, JAM-A
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Type: Antibody
• Monoclonal or Polyclonal: Monoclonal
• Target Species: Human

• Applications: Flow Cytometry
• Clone: CSTEM27
• Conjugate: PE
• Gene: F11R
• Format: Liquid
• Gene Symbols: F11R
• Isotype: IgG2a, kappa
• Quantity: 100 Tests
• Storage Requirements: 4° C, store in dark, DO NOT FREEZE!
• Primary or Secondary: Primary
• Gene ID (Entrez): 50848