CD32b Monoclonal Antibody (AT130-2), APC, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Supplier:  LIFE TECHNOLOGIES 17032182

Catalog No. 50-112-3043

Description

Description: The monoclonal antibody AT130-2 reacts with mouse CD32b, also known as Fc-gamma receptor IIb. CD32b is the only inhibitory member of the Fc-gamma receptor family. Immune complex engagement of CD32b results in the phosphorylation of its intracellular ITIM motif. This engagement serves to attenuate B cell receptor (BCR) signaling and other activating signals, reduces proliferation, reduces antibody production, and may also deliver apoptotic signals. CD32b is the only Fc-gamma receptor expressed by B cells. CD32b is also expressed on monocytes, dendritic cells, neutrophils, eosinophils and basophils, as well as B cell progenitors and plasma cells. AT130-2 has been reported to have agonist activity. Applications Reported: This AT130-2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This AT130-2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser. Filtration: 0.2 μm post-manufacturing filtered.

This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants.

Specifications

• Antigen: CD32b
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Formulation: PBS with 0.09% sodium azide; pH 7.2
• Gene Accession No.: P08101
• Gene Symbols: FCGR2B
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 14130
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid
• Green Features: Responsibly packaged

• Applications: Flow Cytometry
• Clone: AT130-2
• Conjugate: APC
• Gene: FCGR2B
• Gene Alias: AI528646; CD32; CD32 receptor 2; CD32 receptor 2a; CD32 receptor 2b; CD32A; CD32B; CDw32; F630109E10Rik; Fc fragment of IgG receptor IIb; Fc fragment of IgG, low affinity II, receptor for (CD32); Fc fragment of IgG, low affinity IIb, receptor; Fc fragment of IgG, low affinity IIb, receptor (CD32); Fc fragment of IgG, low affinity IIb, receptor for (CD32); fc gamma receptor IIB; Fc gamma RIIb; Fc receptor, IgG, low affinity IIb; Fc[g]RII; FCG2; FC-gamma RII; fc-gamma RII-a; Fc-gamma RII-b; Fc-gamma-RII; fc-gamma-RIIa; FcgammaRIIB; fc-gamma-RIIb; FcgammaRIIB2; FcGR; Fcgr2; Fcgr2a; FCGR2A1; FCGR2B; FcgRII; Fcr-2; Fcr-3; fcRII; fcRII-a; fcRII-b; FGFR2B; IGFR2; IgG Fc receptor II beta; igG Fc receptor II-b; Low affinity immunoglobulin gamma Fc region receptor II; low affinity immunoglobulin gamma Fc region receptor II-b; Ly-17; LyM-1; Ly-m20; Lymphocyte antigen 17
• Host Species: Mouse
• Quantity: 100 μg
• Primary or Secondary: Primary
• Target Species: Mouse
• Product Type: Antibody
• Isotype: IgG2a κ

Safety and Handling

WARNING: Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.