CD33, Mouse anti-Human, Clone: P67.6, Millipore Sigma™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
MABF216325	MilliporeSigma MABF216325UG	25 μg
MABF2163100	MilliporeSigma MABF2163100UG	100 μg

Description

Anti-CD33, clone P67.6, Cat. No. MABF2163, is a mouse monoclonal antibody that detects CD33 and has been tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition assays.

Myeloid cell surface antigen CD33 (UniProt: P20138; also known as Sialic acid-binding Ig-like lectin 3, Siglec-3, gp67, CD33) is encoded by the CD33 (also known as SIGLEC3) gene (Gene ID: 945) in human. CD33 is a single-pass type I membrane sialoadhesion protein that is expressed by myeloid stem cells, myeloblasts, monoblasts, monocytes, macrophages, and granulocyte precursors and preferentially binds to alpha-2,6-linked sialic acid. CD33 is not expressed in erythrocytes, platelets, B-cells, T-cells, and NK cells. Hence, it can serve as a marker for myeloid cells. It is synthesized with a signal peptide (aa 1-17), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 18-259), a transmembrane domain (aa 260-282), and a cytoplasmic domain (aa 283-364). CD33 contains two copies of a cytoplasmic motif (aa 338-343 and 356-361), which is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. In the immune response, CD33 may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. Human CD33 is reported to contain two tyrosine residues in its cytoplasmic domain (tyrosine 340 and 358) that when phosphorylated could function as docking sites for the phosphatases, SHP-1 and/or SHP-2, and enable CD33 to function as an inhibitory receptor.

Specifications

• Antigen: CD33
• Dilution: Inhibition Assays: A representative lot of CD33 conjugated to calicheamicin inhibited inhibits the formation of colonies from AML marrow samples. (Hamann, P.R., et. al. (2002). Bioconjug Chem. 13(1):47-58). Flow Cytometry Analysis: A representative lot detected CD33 in Flow Cytometry applications (Kussick, S.J., et. al. (2003). Arch Pathol Lab Med. 127(9):1140-7).Immunocytochemistry Analysis: A representative lot detected CD33 in Immunocytochemistry applications (van Der Velden, V.H., et. al. (2001). Blood. 97(10):3197-204).
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human
• Gene Alias: Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67
• Gene Symbols: CD33;SIGLEC3
• Isotype: IgG1 κ
• Purification Method: Protein G purified
• Test Specificity: Clone P67.6 specifically detects human CD 33.

• Clone: P67.6
• Applications: Flow Cytometry, Immunocytochemistry, Inhibition Assays
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Inflammation & Immunology
• Formulation: Purified mouse monoclonal antibody IgG1 in PBS without azide.
• Gene ID (Entrez): NP_001763.3
• Immunogen: FMY9S5 cells expressing CD33.
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.