CD335 (NKp46) Monoclonal Antibody (9E2), Functional Grade, eBioscience™, Invitrogen™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
16-335-982	Invitrogen™ 16335982	100 μg
16-335-985	Invitrogen™ 16335985	500 μg

Description

This 9E2 monoclonal antibody reacts with human CD335, which is also known as NKp46. Expressed on NK cells, CD335 is a member of the natural cytotoxicity receptor family that also includes NKp30 and NKp44. This type I transmembrane glycoprotein is involved in activating and expanding resting NK cells following interaction with dendritic cells to eliminate tumor and virus-infected (e.g., influenza) cells. CD335 also interacts with pancreatic beta cells during the development of type I diabetes. The 9E2 antibody has been reported to induce cytotoxicity. Applications Reported: This 9E2 antibody has been reported for use in flow cytometric analysis and to induce cytotoxicity in functional assays. Applications Tested: This 9E2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount ( μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Storage and handling: Use in a sterile environment. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/ μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

NKp46 (CD335, NCR1, ) is a cytotoxicity-activating receptor that may contribute to the increased efficiency of activated natural killer (NK) cells to mediate tumor cell lysis. NKp46 consists of two Ig-like domains assembled to leukocyte immunoglobulin-like (LIR) and killer inhibitory receptors (KIR).

Specifications

• Antigen: CD335 (NKp46)
• Concentration: 1 mg/mL
• Classification: Monoclonal
• Form: Liquid
• Regulatory Status: RUO
• Formulation: PBS with no preservative; pH 7.2
• Gene ID (Entrez): 9437
• Primary or Secondary: Primary
• Content And Storage: 4°C
• Gene: NCR1

• Clone: 9E2
• Applications: Flow Cytometry, Functional Assay
• Conjugate: Functional Grade
• Host Species: Mouse
• Target Species: Human
• Gene Accession No.: O76036
• Isotype: IgG1 κ
• Purification Method: Affinity chromatography
• Product Type: Antibody