Invitrogen™ CD34 Monoclonal Antibody (1H6), PE, eBioscience™, Invitrogen™

Catalog No.	Quantity
50-103-31	100 Tests
50-103-30	25 Tests

Catalog No. 50-103-31 Supplier Invitrogen™ Supplier No. 12034042

Description

Mouse Monoclonal Antibody

Description: The 1H6 monoclonal antibody reacts with canine CD34, also known as mucosialin. CD34 belongs to a protein family that also includes endoglycan and podocalyxin. These single-pass transmembrane proteins possess heavily glycosylated extracellular and N-terminal mucin domains. Similar to humans, canine CD34 can be detected on hematopoietic progenitor cells in the bone marrow, peripheral blood and hair follicle, as well as on some populations of mesenchymal stem cells, tumor cell lines, and on vascular endothelia in the adult. CD34 can be used as a marker to isolate cells capable of hematopoietic cell engraftment. The 1H6 antibody has been reported to work for enriching CD34+ cells. Applications Reported: This 1H6 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 1H6 antibody has been pre-titrated and tested by flow cytometric analysis of a canine CD34-expressing cell line. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 μm post-manufacturing filtered.

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

Specifications

• Antigen: CD34
• Applications: Flow Cytometry
• Classification: Monoclonal
• Clone: 1H6
• Concentration: 5 μL/Test
• Conjugate: PE
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene: Cd34
• Gene Accession No.: Q28270
• Gene Alias: (E3-independent) E2 ubiquitin-conjugating enzyme R1; AU040960; CD34; CD34 antigen; CD34 molecule; CDC34; cell division cycle 34; cell division cycle 34 homolog; cluster designation 34; cluster of differentiation antigen 34; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; Mucosialin; RP11-328D5.2; sialomucin protein; type 1 transmembrane protein; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
• Gene Symbols: Cd34
• Host Species: Mouse
• Purification Method: Affinity chromatography
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 415130
• Target Species: Canine
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG1

Safety and Handling

WARNING: Cancer and Reproductive Harm - www.P65Warnings.ca.gov

For Research Use Only.