Invitrogen™ CD34 Monoclonal Antibody (QBEND/10)

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ MA516926

Catalog No. PIMA516926

Description

This antibody does not react with bovine, dog, rat or sheep. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. A suggested positive control for immunohistochemical applications is tonsil or bone marrow. This product does not require antigen retreival using heat treatment or protein digestion. However staining of paraffin-embedded, formalin-fixed tissues may be enhanced by enzyme pre-treatment using pronase or heat treatment using citrate buffer. Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34.

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

Specifications

• Antigen: CD34
• Classification: Monoclonal
• Concentration: 1 mg/mL
• Formulation: PBS with 0.09% sodium azide
• Gene Accession No.: P28906
• Gene Symbols: Cd34
• Immunogen: Human endothelial cell membrane vesicles.
• Quantity: 200 μg
• Primary or Secondary: Primary
• Target Species: Human, Monkey
• Product Type: Antibody
• Isotype: IgG1

• Applications: Flow Cytometry, Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunomicroscopy, Immunoprecipitation, Western Blot, Immunocytochemistry
• Clone: QBEND/10
• Conjugate: Unconjugated
• Gene: Cd34
• Gene Alias: (E3-independent) E2 ubiquitin-conjugating enzyme R1; AU040960; CD34; CD34 antigen; CD34 molecule; CDC34; cell division cycle 34; cell division cycle 34 homolog; cluster designation 34; cluster of differentiation antigen 34; E2 ubiquitin-conjugating enzyme R1; E2-CDC34; hematopoietic progenitor cell antigen CD34; hematopoietic progenitor cell marker CD34; Mucosialin; RP11-328D5.2; sialomucin protein; type 1 transmembrane protein; UBC3; UBCH3; UBE2R1; ubiquitin carrier protein; ubiquitin conjugating enzyme E2 R1; ubiquitin-conjugating enzyme E2 R1; Ubiquitin-conjugating enzyme E2-32 kDa complementing; ubiquitin-conjugating enzyme E2-CDC34; Ubiquitin-protein ligase R1
• Host Species: Mouse
• Purification Method: Protein A
• Regulatory Status: RUO
• Gene ID (Entrez): 947
• Content And Storage: 4°C, do not freeze
• Form: Liquid