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CD357 (AITR/GITR) Rat anti-Mouse, eFluor® 450, Clone: DTA-1, eBioscience™ 
Rat Monoclonal Antibody
$154.00 - $305.00
Specifications
| Antigen | CD357 (AITR/GITR) |
|---|---|
| Clone | DTA-1 |
| Target Species | Mouse |
| Conjugate | eFluor™ 450 |
| Isotype | IgG2b, kappa |
Description
Description: The DTA-1 monoclonal antibody reacts with mouse GITR, Glucocorticoid-Induced TNFR family gene, also known as TNFRSF18. In naive mice, GITR is expressed predomitly by CD4+CD25+ T regulatory cells (Treg) and by CD25+ CD4+ CD8- thymocytes. Stimulation of GITR with DTA-1 antibody abrogates Treg cell-mediated suppression. Although it does not deplete CD357 expressing cells in vivo, it has been shown to induce tumorogenesis. The removal of GITR-expressing Treg cells or the administration of DTA-1 resulted in organ specific autoimmune disease. Applications Reported: This DTA-1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This DTA-1 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.
2 µm post-manufacturing filtered. Glucocorticoid-induced tumor necrosis factor receptor family-related gene (GITR) is a member of the tumor necrosis factor receptor (TNFR) family that is expressed at low levels on unstimulated T cells, B cells, and macrophages. Upon activation, CD4+ and CD8+ T cells up-regulate GITR expression, whereas immunoregulatory T cells constitutively express high levels of GITR.Specifications
| CD357 (AITR/GITR) | |
| Mouse | |
| IgG2b, kappa | |
| Monoclonal | |
| Liquid | |
| Antibody | |
| 4° C, store in dark, DO NOT FREEZE! |
| DTA-1 | |
| eFluor™ 450 | |
| Flow Cytometry | |
| Primary | |
| Monoclonal | |
| PBS with 0.1% gelatin and 0.09% sodium azide; pH 7.2 | |
| RUO |
For Research Use Only.
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