CD366 (TIM-3) Mouse anti-Mouse, PE, Clone: 5D12/TIM-3 (also known as 5D12), BD

Mouse Monoclonal Antibody

Manufacturer:  BD Biosciences 566346

Catalog No. BDB566346

Description

The 5D12 monoclonal antibody specifically binds to NKp80 which is also known as C-type lectin domain family 5 member C (CLEC5C). NKp80 is a single-pass type II transmembrane glycoprotein that contains a C-type lectin extracellular domain and is expressed as a ñ80 kDa disulfide-linked homodimer. NKp80 is encoded by KLRF1 /(Killer cell lectin-like receptor subfamily F member 1) which maps to the NK-gene complex (NKC). NKp80 is expressed on most NK cells, and subsets ofγδ T cellsand effector-memory CD8αβT cells. NKp80 is not present in rodents. NKp80 is an activating coreceptor that induces NK cell-mediated cytotoxicity and proinflammatory cytokine (eg, IFN-γand TNF) production upon interaction with its ligand, AICL (Activation induced C type lectin). AICL is encoded by CLEC2B /that is selectively expressed on myeloid cells including activated monocytes, macrophages, and granulocytes. NKp80 can reportedly transduce signals through an atypical hemi-ITAM-like sequence motif and Syk kinase to trigger cellular responses.

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Specifications

• Antigen: CD366 (TIM-3)
• Concentration: 0.2mg/mL
• Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
• Gene Alias: Cd366, T-cell membrane protein, Tim3, TIMD-3, Timd3, HAVcr-2, Havcr2,
• Immunogen: Mouse TIM-3 Recombinant Protein
• Purification Method: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography. The antibody was conjugated with R-PE under optimum conditions, and unconjugated antibody and free PE were removed.
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Murine

• Clone: 5D12/TIM-3 (also known as 5D12)
• Conjugate: PE
• Format: Affinity Purified
• Host Species: Murine
• Isotype: Mouse IgG₁, κ
• Quantity: 100μg
• Storage Requirements: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
• Monoclonal or Polyclonal: Monoclonal