CD366 (TIM3) Rat anti-Mouse, Clone: 8B.2C12, eBioscience
Rat Monoclonal Antibody
Manufacturer: Invitrogen 14587185
Description: The 8B.2C12 monoclonal antibody reacts with mouse CD366 (TIM3), a Th1-specific cell surface protein. CD366 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. CD366 is expressed selectively by differentiated CD4+ Th1 and CD8+ Tc1, but is absent on Th2 and Tc2. Other hematopoietic cell types, including naive T cells, B cells, macrophages and dendritic cells, do not express CD366, at least at the protein level. Expression of CD366 is upregulated at a late stage of T cell differentiation on Th1 cells after 3 rounds of in vitro polarization suggesting a role for this molecule in the transport or effector function of Th1 cells rather than a contribution to T cell differentiation. In an experimental autoimmune encephalomyelitis (EAE) model, CD366 was shown to be expressed on most CD4+ and CD8+ T cells in the central nervous system at the onset of clinical signs of disease, while less than 2% of CD4+ cells in the periphery expressed CD366 after immunization. In this model, in vivo administration of 8B.2C12 resulted in a hyperacute and atypical disease phenotype. It is postulated that the engagement of CD366 during T cell activation results in the expansion and activation of macrophages and increased severity of autoimmune disease. The Tim gene family may have an important role in the regulation of autoimmunity and allergies.The 8B.2C12 antibody binds to the BALB/c allele of CD366 while reactivity to the C57Bl/6 allele is significantly weaker. Applications Reported: The 8B.2C12 antibody has been reported for use in flow cytometric analysis, and immunoprecipitation. It has also been reported for use in functional assays. (Please use Functional Grade purified 8B.2C12, cat. 16-5871, in functional assays.). Applications Tested: The 8B.2C12 antibody has been tested by flow cytometric analysis of mouse splenocytes and mouse CD366 transfected cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered. TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.
|PBS with 0.09% sodium azide; pH 7.2|
|Flow Cytometry, Immunohistochemistry, Immunoprecipitation|
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