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  6. Invitrogen™ Mouse IgD Monoclonal Antibody (11-26c (11-26)), NovaFluor™ Violet 800 

Invitrogen™ Mouse IgD Monoclonal Antibody (11-26c (11-26)), NovaFluor™ Violet 800

Catalog No. 503126233
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Catalog No. Quantity
50-312-6233 25 μg
M030T03V03A 100 μg
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Catalog No. 50-312-6233 Supplier Invitrogen™ Supplier No. M030T02V03A
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Description

Rat Monoclonal Antibody

Description The 11-26c monoclonal antibody reacts with the delta heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells. This product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer. Applications Tested This 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.4 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Master mixes Master mixes of NFs should be made at 2-8 °C and may be made up to 4 hours ahead of time. We do not recommend storing master mixes containing NovaFluor conjugates overnight or longer. Whole Blood compatibility When utilizing whole blood (as opposed to density-gradient-purified PBMC), we recommend lysing red blood cells in bulk prior to staining with NovaFluor conjugates. See the Bulk Lysis of Human Whole Blood protocol here. Staining of whole blood with NovaFluor conjugates followed by lysis of red blood cells may result in higher-than-expec...

This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.
TRUSTED_SUSTAINABILITY

Specifications

Antigen Mouse IgD
Applications Flow Cytometry
Classification Monoclonal
Clone 11-26c (11-26)
Conjugate NovaFluor Violet 800
Formulation PBS with BSA and 0.09% sodium azide; pH 7.2
Gene Ighd
Gene Accession No. P01881
Gene Alias IgD; Igh-5; IGHD; immunoglobulin heavy constant delta
Gene Symbols Ighd
Host Species Rat
Purification Method Affinity chromatography
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Gene ID (Entrez) 380797
Target Species Mouse
Content And Storage 4°C, store in dark, DO NOT FREEZE!
Product Type Antibody
Form Liquid
Isotype IgG2a κ
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