Invitrogen™ CD3e/CD4 Antibody Cocktail, FITC, PE

Catalog No.	Quantity
PIMA516607	100 Tests

Catalog No. PIMA516607 Supplier Invitrogen™ Supplier No. MA516607

Description

Antibody Cocktail

CD3e FITC reagent: IgG1 (mouse). CD4 RPE reagent: IgG2a (rat). Clone CA17.2A12 recognizes canine CD3 epsilon, a T cell differentiation antigen expressed on mature T lymphocytes. Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils. For FACS analysis, use 10 μL of the suggested working dilution to label 1x10^6 cells in 100 μL. Reconstitute with 1 mL distilled water. After reconstitution, store product undiluted at 4°C in the dark.

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

Specifications

• Antigen: CD3e/CD4
• Applications: Flow Cytometry
• Classification: Cocktail
• Clone: CA17.2A12/YKIX302.9
• Concentration: Conc. Not Determined
• Conjugate: FITC/PE
• Formulation: PBS with 1% BSA, 5% sucrose and 0.09% sodium azide
• Gene: CD4
• Gene Accession No.: P27597, P33705
• Gene Alias: Cd247; CD247 antigen; CD247 antigen, zeta subunit; CD247 molecule; CD3 antigen delta polypeptide; CD3 antigen, delta subunit; CD3 antigen, epsilon polypeptide; CD3 antigen, gamma polypeptide; CD3 antigen, zeta polypeptide; CD3 delta; CD3 epsilon; CD3 gamma-chain; CD3 molecule delta polypeptide; CD3 molecule, delta; CD3 molecule, epsilon; CD3 molecule, epsilon polypeptide; CD3 molecule, gamma; CD3 molecule, gamma polypeptide; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule; CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; Cd3e; CD3E antigen, epsilon polypeptide (TiT3 complex); CD3e molecule; CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; Cd3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule; CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3-GAMMA; CD3H; CD3Q; Cd3z; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3-ZETA; CD3zeta chain; Cd4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; CD4 molecule; CD4 receptor; CD4mut; FLJ18683; IMD17; IMD18; IMD19; IMD25; Leu-2; MAL; OKT3, delta chain; OKT8 T-cell antigen; p55; T cell co-receptor; T3D; T3E; T3G; T3Z; T8 T-cell antigen; T-cell antigen Leu2; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor CD3 subunit zeta; T-cell receptor CD3, subunit zeta; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T-cell surface glycoprotein CD4; TCR zeta chain; TCRE; TCRZ; TCRzeta; W3/25; W3/25 antigen
• Gene Symbols: CD247, CD3D, CD3E, CD3G, CD4
• Host Species: Mixed
• Immunogen: Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-9, Canine Concanavilin A activated T-cell blasts.
• Purification Method: Purified
• Quantity: 100 Tests
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 403931, 442981, 479419, 489383, 611571
• Target Species: Canine
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Product Type: Antibody
• Form: Lyophilized
• Isotype: IgG1, IgG2a