CD4/CD25 Antibody Cocktail, APC, FITC, eBioscience™, Invitrogen™

Antibody Cocktail

Supplier:  Invitrogen 22042571

Catalog No. 50-154-16

Description

Description: This product consists of two fluorochrome-conjugated monoclonal antibodies to identify CD4+CD25+ human peripheral blood cells. The RPA-T4 monoclonal antibody (mouse IgG1, kappa) reacts with human CD4, a 59 kDa cell surface receptor that is expressed by a majority of thymocytes, a subpopulation of mature T cells (T helper cells) and at low levels on monocytes. The BC96 monoclonal antibody (mouse IgG1, kappa) reacts with human CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Ralpha). This cocktail of RPA-T4 and BC96 can be used for the flow cytometric detection of human regulatory T cells, which also express Foxp3. Applications Reported: This product has been reported for use in flow cytometric analysis. Applications Tested: This cocktail has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μL per test (containing 1 μg of Anti-Human CD4 (RPA-T4) FITC and 0.125 μg of Anti-Human CD25 (BC96) APC). A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix. CD25: IL2 receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains (IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternately-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown. Mutations in this gene are associated with interleukin 2 receptor alpha deficiency.

Specifications

• Antigen: CD4/CD25
• Classification: Cocktail
• Concentration: 20 μL/Test
• Formulation: PBS with 0.2% BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P01589, P01730
• Gene Symbols: CD4, IL2RA
• Purification Method: Affinity chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 3559, 920
• Content And Storage: 4° C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: RPA-T4, BC96
• Conjugate: FITC
• Gene: CD4
• Gene Alias: CD25; CD4; CD4 antigen; CD4 antigen (p55); CD4 antigen p55; CD4 molecule; CD4 receptor; CD4mut; IDDM10; IL 2 RA; IL 2 receptor; IL 2R; IL 2R subunit alpha; IL2 RA; IL2 RA antibody; IL2 receptor; IL-2 receptor alpha subunit; IL2 receptor subunit alpha; IL-2 receptor subunit alpha; Il2r; IL-2R alpha; IL-2R alpha chain; IL2R subunit alpha; IL-2R subunit alpha; Il2ra; IL-2-RA; IL2-RA; Il2ra antibody; IL2RAC; IMD41; interleukin 2 receptor subunit alpha; interleukin 2 receptor, alpha; interleukin 2 receptor, alpha chain; interleukin-2 receptor alpha chain; Interleukin2 receptor subunit alpha; interleukin-2 receptor subunit alpha; L3T4; Ly-4; Ly-43; p55; p55 chain; sIL 2R; soluble IL 2 receptor; TAC antigen; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); TCGFR; W3/25; W3/25 antigen
• Host Species: Mouse
• Quantity: 25 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ

For Research Use Only.