Learn More
Invitrogen™ CD42a Monoclonal Antibody (GR-P), eFluor™ 450, eBioscience™, Invitrogen™
Description
Description: This GR-P monoclonal antibody reacts with human CD42a, which is also known as glycoprotein IX (GPIX). CD42a is a 22-kDa transmembrane protein that is constitutively expressed on platelets and megakaryocytes. CD42a associates with CD42b, CD42c, and CD42d to form the GPIb-V-IX complex, which binds the von Willebrand factor, an endothelial adhesion protein, to mediate platelet adhesion and activation. This binding results in platelet aggregation at sites of vascular injury. This receptor complex has also been demonstrated to bind thrombin, factors XI and XII, P-selectin, and Mac-1. Finally, a point mutation within the leucine-rich motif of CD42a has been linked to Bernard-Soulier syndrome. Applications Reported: Anti-Human CD42a eFluor™ 450 has been reported for use in flow cytometric analysis. Applications Tested: The GR-P antibody has been pre-titrated and tested by flow cytometric analysis of normal human platelets. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor™ 450 is an alternative to Pacific Blue™. eFluor™ 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.
Specifications
Specifications
| Antigen | CD42a |
| Applications | Flow Cytometry |
| Classification | Monoclonal |
| Clone | GR-P |
| Concentration | 5 μL/Test |
| Conjugate | eFluor 450 |
| Formulation | PBS with BSA and 0.09% sodium azide; pH 7.2 |
| Gene | GP9 |
| Gene Accession No. | P14770 |
| Gene Alias | CD42a; Glycoprotein 9; glycoprotein 9 (platelet); glycoprotein IX (platelet); glycoprotein IX platelet; GP9; GPIX; GP-IX; platelet glycoprotein IX; Platelet GPIX |
| Show More |
Frequently Asked Questions (FAQs)
Our options will depend on the samples you are analyzing.
If cell viability is not critical, you can store your stained samples at 4 degrees C or on ice overnight in the dark and analyze the following day.
For samples stained with eFluor organic fluorochromes, we recommend that cells be suspended in 100 uL of Flow Cytometry Staining Buffer (Cat. No. 00-4222) and 100 uL of eBioscience IC Fixation Buffer (Cat. No. 00-8222); samples can be incubated for up to 3 days at 4 degrees C in the dark. Alternatively, the 1-step Fix/Lyse Solution (Cat. No. 00-5333) can be used. This is a great option when working with whole blood but also works for other cell types.
Yes, the eFluor Organic fluorochromes can be used for intracellular staining. The eFluor organic fluorochromes maintain bright signal and require minimal changes in compensation when fixed with eBioscience IC Fixation Buffer (Cat. No. 00-8222-49) and Permeabilization Buffer (Cat. No. 00-8333-56) or 1-step Fix/Lyse Solution (Cat. No. 00-5333-54, 00-5333-57) (as compared to live cells).
As with other fluorochromes, we recommend minimal exposure to light to maintain optimal signal.
For Research Use Only.
By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.