Invitrogen™ CD42a Monoclonal Antibody (GR-P), FITC, eBioscience™

Mouse Monoclonal Antibody

Supplier:  Invitrogen™ 11042842

Catalog No. 50-245-235

Description

Description: This GR-P monoclonal antibody reacts with human CD42a, which is also known as glycoprotein IX (GPIX). CD42a is a 22-kDa transmembrane protein that is constitutively expressed on platelets and megakaryocytes. CD42a associates with CD42b, CD42c, and CD42d to form the GPIb-V-IX complex, which binds the von Willebrand factor, an endothelial adhesion protein, to mediate platelet adhesion and activation. This binding results in platelet aggregation at sites of vascular injury. This receptor complex has also been demonstrated to bind thrombin, factors XI and XII, P-selectin, and Mac-1. Finally, a point mutation within the leucine-rich motif of CD42a has been linked to Bernard-Soulier syndrome. Applications Reported: This GR-P antibody has been reported for use in flow cytometric analysis. Applications Tested: This GR-P antibody has been pre-titrated and tested by flow cytometric analysis of human platelets. This can be used at 5 μL (0.25 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser. Filtration: 0.2 μm post-manufacturing filtered.

Single-chain membrane glycoprotein that forms a non-covalent complex with GPIb. (MW 23 kDa) Reactivity with resting and activated platelets, weakly on monocytes, megakaryocytes and attachment site for the platelet plasma membrane to the submembrane cytoskeleton. GPIb/IX complex, functions as the receptor for ristocetin-induced binding of von Willebrand factor and as the von Willebrand factor-depend adhesion receptor.

Specifications

• Antigen: CD42a
• Classification: Monoclonal
• Concentration: 5 μL/Test
• Formulation: PBS with BSA and 0.09% sodium azide; pH 7.2
• Gene Accession No.: P14770
• Gene Symbols: GP9
• Purification Method: Affinity Chromatography
• Regulatory Status: RUO
• Gene ID (Entrez): 2815
• Content And Storage: 4°C, store in dark, DO NOT FREEZE!
• Form: Liquid

• Applications: Flow Cytometry
• Clone: GR-P
• Conjugate: FITC
• Gene: GP9
• Gene Alias: CD42a; Glycoprotein 9; glycoprotein 9 (platelet); glycoprotein IX (platelet); glycoprotein IX platelet; GP9; GPIX; GP-IX; platelet glycoprotein IX; Platelet GPIX
• Host Species: Mouse
• Quantity: 100 Tests
• Primary or Secondary: Primary
• Target Species: Human
• Product Type: Antibody
• Isotype: IgG1 κ