CD44 Std./H-CAM Ab-4 Mouse Monoclonal Antibody, Epredia™

Ensure accurate, reproducible results in immunohistochemistry, western blotting, immunoprecipitation and flow cytometry experiments.

Catalog Number	Mfr. No.	Form	Conjugate	Quantity
MS668P1ABX	Epredia MS668P1ABX	Concentrated	Unconjugated	100 μL
MS668P1	Epredia MS668P1	Concentrated	Unconjugated	500 μL
MS668P	Epredia MS668P	Concentrated	Unconjugated	1 mL
MS668B0	Epredia MS668B0	Concentrated	Biotin	100 μL
MS668B1	Epredia MS668B1	Concentrated	Biotin	500 μL
MS668B	Epredia MS668B	Concentrated	Biotin	1 mL
MS668PABX	Epredia MS668PABX	Concentrated	Unconjugated	200 μL
MS668R1	Epredia MS668R1	Prediluted	Unconjugated	1 mL

Description

CD44 is a cell surface glycoprotein expressed on lymphocytes, monocytes, and granulocytes. CD44 is also known as homing cell adhesion molecule (H-CAM), Phagocytic glycoprotein-1 (PgP-1), ECM-III, HUTCH-1, or Hermes-1. CD44 has been implicated in cell migration, in lymphocyte homing, and in tumor metastasis. Ab-4 reacts with red cells pretreated with 2-aminoethylisothiouronium bromide (AET). Digestion of intact erythrocytes by trypsin treatment leaves a 50-54 kDa protein fragment attached to the membrane, while chymotrypsin leaves a somewhat similarly sized 51-56 kDa component that are recognized by Ab-4. This antibody enhances IL-2 production by CD2-transfected murine hybridoma stimulated by JY cells while it has no significant effect on IL-2 production by Jurkat cells stimulated with CD3, PMA, or CD2. Ab-4 enhances proliferation of PBL stimulated with CD2.

Host Species: Mouse

Clone: 156-3C11

Isotype: IgG2a

Species Reactivity: Human, Baboon and Green Monkey. Does not react with rat. Others not tested.

Epitope: Resistant to digestion by trypsin and chymotrypsin

Immunogen: Stimulated human leukocytes

Molecular Weight: 80-95 kDa

Positive Control: HUVEC or HeLa cells, tonsil or esophageal CA

Cellular Localization: Membrane

Recommended for:

• Immunohistochemistry (Formalin/paraffin)
• Western Blotting
• Immunoprecipitation (Native verified)
• Flow Cytometry

Specifications

• Antigen: CD44 Std./H-CAM Ab-4
• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Western Blot
• Host Species: Mouse
• Research Discipline: Oncogenesis
• Immunogen: Stimulated human leukocytes
• Primary or Secondary: Primary

• Clone: 156-3C11
• Classification: Monoclonal
• Regulatory Status: IVD
• Target Species: Human, Non-human Primate
• Isotype: IgG2a