Invitrogen™ CD45 Monoclonal Antibody (30-F11), Pacific Orange™

Catalog No.	Quantity
MCD4530	1 mL

Catalog No. MCD4530 Supplier Invitrogen™ Supplier No. MCD4530

Description

Rat Monoclonal Antibody

Applications reported for the Rat anti-Mouse CD45 mAb include flow cytometry, immunohistochemistry (zinc- and formalin-fixed, paraffin-embedded), and immunohistochemistry (acetone-fixed, frozen). If the reagent is being diluted, it is recommended that only the quantity to be used within one week be diluted. Cells should be analyzed within 18 hours of staining for best results. Avoid light exposure with fluorochrome-conjugated antibodies. Use dim light during handling, incubation with cells, and prior to analysis. For Pacific Orange™ conjugates: excitation 405 nm/peak emission 551 nm. Matching isotype control is available: Rt IgG2b Pacific Orange™.

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

Specifications

• Antigen: CD45
• Applications: Flow Cytometry, Immunohistochemistry, Immunocytochemistry
• Classification: Monoclonal
• Clone: 30-F11
• Conjugate: Pacific Orange
• Formulation: PBS with 0.1% sodium azide
• Gene: PTPRC
• Gene Accession No.: P06800
• Gene Alias: B220; cd45; CD45 antigen; CD45 antigen isoform 1 precursor; CD45 antigen isoform 2 precursor; CD45 antigen isoform 3 precursor; CD45 antigen isoform 4 precursor; CD45 antigen isoform 5 precursor; CD45 antigen isoform 6 precursor; CD45R; GP180; Lca; L-CA; leucocyte common antigen; leukocyte common antigen; leukocyte common antigen A; leukocyte common antigen B; leukocyte common antigen, CD45; loc; LOW QUALITY PROTEIN: receptor-type tyrosine-protein phosphatase C; LY5; Ly-5; lymphocyte antigen 5; lymphocyte common antigen; Lyt-4; membrane tyrosine phosphatase; protein tyrosine phosphatase lambda; protein tyrosine phosphatase receptor type C; protein tyrosine phosphatase, receptor type C; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Protein tyrosine phosphatase, receptor-type, c polypeptide; protein tyrosine phosphatase; alternatively spliced; Ptprc; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220
• Gene Symbols: PTPRC
• Host Species: Rat
• Immunogen: The CD45 antigen.
• Purification Method: Purified
• Quantity: 1 mL
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Gene ID (Entrez): 19264
• Target Species: Mouse
• Content And Storage: 4°C, store in dark
• Product Type: Antibody
• Form: Liquid
• Isotype: IgG2b κ